Evidence map›Paper›PMID 31879498›Full record

ArticleJournal of orthopaedics

Development of a novel murine femur fracture and fixation model.

Joey P Johnson, Christopher T Born, Nathan Thomas, Jeremy Truntzer, P Kaveh Mansuripur, Justin Kleiner, Scott T McAlister, Dioscaris Garcia, Sarath Koruprolu

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Article in Journal of orthopaedics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Joey P JohnsonDepartment of Orthopedic Surgery, Loma Linda University, 11406 Loma Linda Drive, Suite 128, Loma Linda, CA, 92354, USA.
Christopher T BornDepartment of Orthopaedic Surgery, Warren Alpert Medical School, Brown University, Providence, RI, 593 Eddy Street, Providence, RI, 02903, USA.
Nathan ThomasWarren Alpert Medical School of Brown University, 222 Richmond Street, Providence, RI, 02912, USA.
Jeremy TruntzerDepartment of Orthopedic Surgery, Stanford University, 291 University Drive, Stanford, CA, 94305, USA.
P Kaveh MansuripurDepartment of Orthopaedic Surgery, Warren Alpert Medical School, Brown University, Providence, RI, 593 Eddy Street, Providence, RI, 02903, USA.
Justin KleinerWarren Alpert Medical School of Brown University, 222 Richmond Street, Providence, RI, 02912, USA.
Scott T McAlisterDepartment of Orthopaedic Surgery, Warren Alpert Medical School, Brown University, Providence, RI, 593 Eddy Street, Providence, RI, 02903, USA.
Dioscaris GarciaDepartment of Orthopaedic Surgery, Warren Alpert Medical School, Brown University, Providence, RI, 593 Eddy Street, Providence, RI, 02903, USA.
Sarath KoruproluDepartment of Orthopaedic Surgery, Warren Alpert Medical School, Brown University, Providence, RI, 593 Eddy Street, Providence, RI, 02903, USA.

Funding

COBRE for Skeletal Health and RepairP20GM104937 · NIGMS · RHODE ISLAND HOSPITAL · PI CHEN, QIAN · 2012 to 2016
$10.8M
NIGMS NIH HHS P20 GM104937
6 · The paper itself

Abstract

backgroundAnimal models have been used for decades to simulate human fractures in the laboratory setting. Fracture models in mice are attractive because they offer a high volume, relatively low-cost method of investigating fracture healing characteristics. We report on the development of a novel murine femur fracture model that is rapid, reproducible and inexpensive.

methodsAs part of a pilot study to investigate the effects of smoking on fracture healing, fifteen 35-43 g twelve-week old female CD-1 mice underwent a novel surgical protocol using direct visualization of femur fracture creation and fixation. Following surgery, mice were sacrificed at 14 days, 28 days and 42 days. After sacrifice, the femora were analyzed using MicroCT and histology to evaluate progression of healing.

resultsOf the 14 mice that survived the surgical procedure (one succumbed to a complication of anesthesia), two lost reduction and did not heal. Histology demonstrated at 14 days 44.1% (SD±2.9%) of callus composed of cartilage. At 28 days there was 19.0% (SD±3.4%) of callus composed of cartilage. At 42 days there was 8.4% (SD±2.6%) callus composed of cartilage (p < 0.005). MicroCT demonstrated that from 14 to 42 days the average callus volume decreased from 101.6 mm

conclusionsOur novel fracture and fixation model is an effective, rapid, reproducible and inexpensive method to simulate a fracture in a laboratory setting. Additionally, our model reliably creates a reproducible progression of radiographic and histological bone healing.

Indexed as

Basic scienceFemur fractureFracture model

Identifiers

PMID31879498
PMCPMC6919350

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.