Evidence map›Paper›PMID 31879355›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2020

Rescue of tomato spotted wilt virus entirely from complementary DNA clones.

Mingfeng Feng, Ruixiang Cheng, Minglong Chen, Rong Guo, Luyao Li, Zhike Feng, Jianyan Wu, Li Xie, Jian Hong, Zhongkai Zhang and 2 more

Open access · greenAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
14.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 92 citations in OpenAlex.

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  7. Development of a Reverse Genetics System for Rice Tiller Inhibition Virus.Methods in molecular biology (Clifton, N.J.) · 2026
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  17. NLRs derepress MED10b- and MED7-mediated repression of jasmonate-dependent transcription to activate immunity.Proceedings of the National Academy of Sciences of the United States of America · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Mingfeng FengDepartment of Plant Pathology, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China.
Ruixiang ChengDepartment of Plant Pathology, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China.
Minglong ChenDepartment of Plant Pathology, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China.
Rong GuoDepartment of Plant Pathology, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China.
Luyao LiDepartment of Plant Pathology, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China.
Zhike FengDepartment of Plant Pathology, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China.
Jianyan WuDepartment of Plant Pathology, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China.
Li XieAnalysis Center of Agrobiology and Environmental Sciences, Zhejiang University, 317502 Hangzhou, People's Republic of China.
Jian HongAnalysis Center of Agrobiology and Environmental Sciences, Zhejiang University, 317502 Hangzhou, People's Republic of China.
Zhongkai ZhangYunnan Provincial Key Laboratory of Agri-Biotechnology, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences, 650223 Kunming, People's Republic of China.
Richard KormelinkLaboratory of Virology, Department of Plant Sciences, Wageningen University, 6708PB Wageningen, The Netherlands.ORCID 0000-0001-7360-1884
Xiaorong TaoDepartment of Plant Pathology, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China; taoxiaorong@njau.edu.cn.ORCID 0000-0003-1259-366X
Nanjing Agricultural University · CNZhejiang University · CNGraduate School Experimental Plant Sciences · NLYunnan Academy of Agricultural Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Negative-stranded/ambisense RNA viruses (NSVs) include not only dangerous pathogens of medical importance but also serious plant pathogens of agronomic importance. Tomato spotted wilt virus (TSWV) is one of the most important plant NSVs, infecting more than 1,000 plant species, and poses major threats to global food security. The segmented negative-stranded/ambisense RNA genomes of TSWV, however, have been a major obstacle to molecular genetic manipulation. In this study, we report the complete recovery of infectious TSWV entirely from complementary DNA (cDNA) clones. First, a replication- and transcription-competent minigenome replication system was established based on 35S-driven constructs of the S

Indexed as

DNA, ComplementaryDNA-Directed RNA PolymerasesHepatitis Delta VirusNicotianaNucleocapsid ProteinsPlant DiseasesRepliconRNA, CatalyticRNA, ViralTospovirusViral ProteinsVirionVirus ReplicationDNA, ComplementaryDNA-Directed RNA PolymerasesNucleocapsid ProteinsRNA, CatalyticRNA, ViralViral Proteinsgenome-length infectious cDNA clonesminirepliconnegative-strand RNA virusreverse genetics systemtomato spotted wilt virus

Identifiers

PMID31879355
PMCPMC6969498
OpenAlexW2998410463

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.