ArticleInternational journal of molecular sciences2019
Screening and Identification of Novel cGAS Homologues Using a Combination of in Vitro and In Vivo Protein Synthesis.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Viral capsid delivery of cGAMP enhances STING-dependent antitumor immune response.bioRxiv : the preprint server for biology · 2026Article
- Biochemical Engineering Perspective on cGAS: From Enzyme Discovery to Potential Industrial Application.Chembiochem : a European journal of chemical biology · 2026Review
- Discovery and biochemical characterization of thermostable glycerol oxidases.Applied microbiology and biotechnology · 2024Article
- Cell-free protein synthesis with technical additives - expanding the parameter space of in vitro gene expression.Beilstein journal of organic chemistry · 2024Article
- The efficient synthesis and purification of 2'3'- cGAMP fromFrontiers in microbiology · 2024Article
- 10th anniversary of discovering cGAMP: synthesis and beyond.Organic chemistry frontiers : an international journal of organic chemistry · 2023Article
- Cell-Free Protein Synthesis for the Screening of Novel Azoreductases and Their Preferred Electron Donor.Chembiochem : a European journal of chemical biology · 2022Article
- The Emerging Role of STING in Insect Innate Immune Responses and Pathogen Evasion Strategies.Frontiers in immunology · 2022Review
- How the Innate Immune DNA Sensing cGAS-STING Pathway Is Involved in Autophagy.International journal of molecular sciences · 2021Review
- Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems.Journal of visualized experiments : JoVE · 2021Article
- A Multi-Enzyme Cascade Reaction for the Production of 2'3'-cGAMP.Biomolecules · 2021Article
- Catalytic Promiscuity of cGAS: A Facile Enzymatic Synthesis of 2'-3'-Linked Cyclic Dinucleotides.Chembiochem : a European journal of chemical biology · 2020Article
- Cytosolic Sensors for Pathogenic Viral and Bacterial Nucleic Acids in Fish.International journal of molecular sciences · 2020Review
- Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation.Science advances · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cyclic GMP-AMP synthase (cGAS) catalyzes the synthesis of the multifunctional second messenger, cGAMP, in metazoans. Although numerous cGAS homologues are predicted in protein databases, the catalytic activity towards cGAMP synthesis has been proven for only four of them. Therefore, we selected five novel and yet uncharacterized cGAS homologues, which cover a broad range in the field of vertebrates. Cell-free protein synthesis (CFPS) was used for a pre-screening to investigate if the cGAS genes originating from higher organisms can be efficiently expressed in a bacterial expression system. As all tested cGAS variants were expressible, enzymes were synthesized in vivo to supply higher amounts for a subsequent in vitro activity assay. The assays were carried out with purified enzymes and revealed vast differences in the activity of the homologues. For the first time, the cGAS homologues from the Przewalski's horse, naked mole-rat, bald eagle, and zebrafish were proven to catalyze the synthesis of cGAMP. The extension of the list of described cGAS variants enables the acquisition of further knowledge about the structural and molecular mechanism of cGAS, potentially leading to functional improvement of the enzyme.
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