ReviewInternational journal of molecular sciences2019
Heterologous and Homologous Expression of Proteins from Haloarchaea: Denitrification as Case of Study.
Review in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Determination ofJournal of the Royal Society, Interface · 2025Article
- Characterization of a family IV esterase from extremely halophilic archaeon Haloarcula japonica.Extremophiles : life under extreme conditions · 2024Article
- Comparative genomics of the highly halophilic Haloferacaceae.Scientific reports · 2024Article
- Cloning, Expression, Characterization and Immobilization of a Recombinant Carboxylesterase from the Halophilic Archaeon,Biomolecules · 2024Article
- 5'-untranslated region sequences enhance plasmid-based protein production inFrontiers in microbiology · 2024Article
- A systematic analysis of affinity tags in the haloarchaeal expression system,Frontiers in microbiology · 2024Article
- Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase fromInternational journal of molecular sciences · 2022Article
- Industrial Biotechnology Based on Enzymes From Extreme Environments.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Role of extremophiles and their extremozymes in biorefinery process of lignocellulose degradation.Extremophiles : life under extreme conditions · 2021Review
- Novel Enzymes From the Red Sea Brine Pools: Current State and Potential.Frontiers in microbiology · 2021Review
- Thermococcus kodakarensis provides a versatile hyperthermophilic archaeal platform for protein expression.Methods in enzymology · 2021Article
- Structural and Kinetic Insights Into the Molecular Basis of Salt Tolerance of the Short-Chain Glucose-6-Phosphate Dehydrogenase FromFrontiers in microbiology · 2021Article
- Microorganisms and Their Metabolic Capabilities in the Context of the Biogeochemical Nitrogen Cycle at Extreme Environments.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Haloarchaea (halophilic microbes belonging to the Archaea domain) are microorganisms requiring mid or even high salt concentrations to be alive. The molecular machinery of these organisms is adapted to such conditions, which are stressful for most life forms. Among their molecular adaptations, halophilic proteins are characterized by their high content of acidic amino acids (Aspartate (Asp) and glumate (Glu)), being only stable in solutions containing high salt concentration (between 1 and 4 M total salt concentration). Recent knowledge about haloarchaeal peptides, proteins, and enzymes have revealed that many haloarchaeal species produce proteins of interest due to their potential applications in biotechnology-based industries. Although proteins of interest are usually overproduced in recombinant prokaryotic or eukaryotic expression systems, these procedures do not accurately work for halophilic proteins, mainly if such proteins contain metallocofactors in their structures. This work summarizes the main challenges of heterologous and homologous expression of enzymes from haloarchaea, paying special attention to the metalloenzymes involved in the pathway of denitrification (anaerobic reduction of nitrate to dinitrogen), a pathway with significant implications in wastewater treatment, climate change, and biosensor design.
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