ArticleAmerican journal of physiology. Cell physiology2020
Bioenergetics underlying single-cell migration on aligned nanofiber scaffolds.
Article in American journal of physiology. Cell physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Deep learning reveals how cells pull, buckle, and navigate fibrous environments.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Electrospun Parallel, Crossed Fibers for Promoting Cell Adhesion and Migration.Materials (Basel, Switzerland) · 2025Article
- Dendritic cell force-migration coupling on aligned fiber networks.Biophysical journal · 2024Article
- A multifunctional quercetin/polycaprolactone electrospun fibrous membrane for periodontal bone regeneration.Materials today. Bio · 2024Article
- Bacterial Pyocyanin Inducible Keratin 6A Accelerates Closure of Epithelial Defect under Conditions of Mitochondrial Dysfunction.The Journal of investigative dermatology · 2023Article
- The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis.Biomolecules · 2023Article
- Actin Filaments Couple the Protrusive Tips to the Nucleus through the I-BAR Domain Protein IRSp53 during the Migration of Cells on 1D Fibers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Driving adult tissue repair via re-engagement of a pathway required for fetal healing.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Sculpting Rupture-Free Nuclear Shapes in Fibrous Environments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Quantitative biophysical metrics for rapid evaluation of ovarian cancer metastatic potential.Molecular biology of the cell · 2022Article
- Mechanoresponsive metabolism in cancer cell migration and metastasis.Cell metabolism · 2021Review
- Cell Fragment Formation, Migration, and Force Exertion on Extracellular Mimicking Fiber Nanonets.Advanced biology · 2021Article
- Detecting Effects of Low Levels of FCCP on Stem Cell Micromotion and Wound-Healing Migration by Time-Series Capacitance Measurement.Sensors (Basel, Switzerland) · 2021Article
- Single Cell Forces after Electroporation.ACS nano · 2021Article
- Recent advances in understanding the role of metabolic heterogeneities in cell migration.Faculty reviews · 2021Review
- An American Physiological Society cross-journal Call for Papers on "Deconstructing Organs: Single-Cell Analyses, Decellularized Organs, Organoids, and Organ-on-a-Chip Models".American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Force-exerting perpendicular lateral protrusions in fibroblastic cell contraction.Communications biology · 2020Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell migration is centrally involved in a myriad of physiological processes, including morphogenesis, wound healing, tissue repair, and metastatic growth. The bioenergetics that underlie migratory behavior are not fully understood, in part because of variations in cell culture media and utilization of experimental cell culture systems that do not model physiological connective extracellular fibrous networks. In this study, we evaluated the bioenergetics of C2C12 myoblast migration and force production on fibronectin-coated nanofiber scaffolds of controlled diameter and alignment, fabricated using a nonelectrospinning spinneret-based tunable engineered parameters (STEP) platform. The contribution of various metabolic pathways to cellular migration was determined using inhibitors of cellular respiration, ATP synthesis, glycolysis, or glucose uptake. Despite immediate effects on oxygen consumption, mitochondrial inhibition only modestly reduced cell migration velocity, whereas inhibitors of glycolysis and cellular glucose uptake led to striking decreases in migration. The migratory metabolic sensitivity was modifiable based on the substrates present in cell culture media. Cells cultured in galactose (instead of glucose) showed substantial migratory sensitivity to mitochondrial inhibition. We used nanonet force microscopy to determine the bioenergetic factors responsible for single-cell force production and observed that neither mitochondrial nor glycolytic inhibition altered single-cell force production. These data suggest that myoblast migration is heavily reliant on glycolysis in cells grown in conventional media. These studies have wide-ranging implications for the causes, consequences, and putative therapeutic treatments aimed at cellular migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.