ArticleMatrix biology : journal of the International Society for Matrix Biology2020
Single cell RNA-sequencing reveals cellular heterogeneity and trajectories of lineage specification during murine embryonic limb development.
Article in Matrix biology : journal of the International Society for Matrix Biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 79 citations in OpenAlex.
- STAID: A Self-Refining Deep Learning Framework for Spatial Cell-Type Deconvolution with Biologically Informed Modeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Comprehensive benchmarking of RNA velocity methods across single-cell datasets.Genome biology · 2026Article
- Species-specific roles of cellular communication network proteins in cartilage development: A comparative study using in vitro chondrogenic models.Journal of cell communication and signaling · 2026Article
- Type E endothelial cells orchestrate embryonic skeletogenesis via chondrogenic priming of skeletal progenitors.Journal of orthopaedic translation · 2026Article
- snRNA sequencing-based skeletal muscle analysis of Jiangquan black pigs with different average daily growth rates.Scientific reports · 2026Article
- Stage- and cluster-specific regulation of chondrogenic gene programs by Sox9 in mouse embryonic limb buds.iScience · 2026Article
- Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model.Science advances · 2025Article
- The roles of TGF-β, Wnt, and MAPK signaling pathways in joint lineage specification in vitro and ex vivo.Stem cell reports · 2025Article
- Comparative single-cell analyses reveal evolutionary repurposing of a conserved gene programme in bat wing development.Nature ecology & evolution · 2025Article
- Evolutionary trends in the emergence of skeletal cell types.Evolution letters · 2025Article
- Single-cell expression profiling of bat wing development.Nature communications · 2025Article
- Divide-and-conquer strategy with engineered ossification center organoids for rapid bone healing through developmental cell recruitment.Nature communications · 2025Article
- A single-cell atlas of the murine limb skeleton integrating the developmental and adult stages.Scientific reports · 2025Article
- Myotube Guidance: Shaping up the Musculoskeletal System.Journal of developmental biology · 2024Review
- Chromatin profiling identifies chondrocyte-specific Sox9 enhancers important for skeletal development.JCI insight · 2024Article
- Transcription factor PBX4 regulates limb development and haematopoiesis in mice.Cell proliferation · 2024Article
- Extracellular matrix protein composition dynamically changes during murine forelimb development.iScience · 2024Article
- Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation.Cell proliferation · 2024Article
- STIGMA: Single-cell tissue-specific gene prioritization using machine learning.American journal of human genetics · 2024Article
- YAP and TAZ couple osteoblast precursor mobilization to angiogenesis and mechanoregulation in murine bone development.Developmental cell · 2024Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The coordinated spatial and temporal regulation of gene expression in the murine hindlimb determines the identity of mesenchymal progenitors and the development of diversity of musculoskeletal tissues they form. Hindlimb development has historically been studied with lineage tracing of individual genes selected a priori, or at the bulk tissue level, which does not allow for the determination of single cell transcriptional programs yielding mature cell types and tissues. To identify the cellular trajectories of lineage specification during limb bud development, we used single cell mRNA sequencing (scRNA-seq) to profile the developing murine hindlimb between embryonic days (E)11.5-E18.5. We found cell type heterogeneity at all time points, and the expected cell types that form the mouse hindlimb. In addition, we used RNA fluorescence in situ hybridization (FISH) to examine the spatial locations of cell types and cell trajectories to understand the ancestral continuum of cell maturation. This data provides a resource for the transcriptional program of hindlimb development that will support future studies of musculoskeletal development and generate hypotheses for tissue regeneration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.