ArticleIn vitro cellular & developmental biology. Animal2020
Establishment and validation of cell pools using primary muscle cells derived from satellite cells of pig skeletal muscle.
Article in In vitro cellular & developmental biology. Animal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
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- Potential of animal-welfare compliant and sustainably sourced serum from pig slaughter blood.Cell and tissue research · 2024Article
- Advancements of 3D bioprinting in regenerative medicine: Exploring cell sources for organ fabrication.Heliyon · 2024Review
- Production of Proliferation- and Differentiation-Competent Porcine Myoblasts for Preclinical Studies in a Porcine Large Animal Model of Muscular Insufficiency.Life (Basel, Switzerland) · 2024Article
- Artificial intelligence and machine learning applications for cultured meat.Frontiers in artificial intelligence · 2024Review
- Transcriptomic Response of Differentiating Porcine Myotubes to Thermal Stress and Donor Piglet Age.International journal of molecular sciences · 2023Article
- A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats.Zoological research · 2023Article
- Investigation of nasal epithelial cells as a surrogate for bronchial epithelial cells in the research of equine asthma.PloS one · 2023Article
- Isolation and Characterization of Primary DMD Pig Muscle Cells as anLife (Basel, Switzerland) · 2022Article
- The effects of temperature and donor piglet age on the transcriptomic profile and energy metabolism of myoblasts.Frontiers in physiology · 2022Article
- Human Cardiac Progenitor Cell-Derived Extracellular Vesicles Exhibit Promising Potential for Supporting Cardiac RepairFrontiers in physiology · 2022Article
- Application of cell culture technology and genetic engineering for production of future foods and crop improvement to strengthen food security.Bioengineered · 2021Review
- Glutamine supplementation stimulates cell proliferation in skeletal muscle and cultivated myogenic cells of low birth weight piglets.Scientific reports · 2021Article
- Effects of temperature on proliferation of myoblasts from donor piglets with different thermoregulatory maturities.BMC molecular and cell biology · 2021Article
- Current Issues and Technical Advances in Cultured Meat Production: A Review.Food science of animal resources · 2021Review
- Berberine Inhibits Telomerase Activity and Induces Cell Cycle Arrest and Telomere Erosion in Colorectal Cancer Cell Line, HCT 116.Molecules (Basel, Switzerland) · 2021Article
- Article
- Replacing Needle Injection by a Novel Waterjet Technology Grants Improved Muscle Cell Delivery in Target Tissues.Cell transplantationArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primary cell cultures derived from satellite cells of skeletal muscle provide an appropriate in vitro model for proliferating myoblasts and differentiating myotubes for muscle biological research. These cell cultures may consist of harvested cells per animal or of a cell pool made of cells from several animals. However, cell pooling reduces the biological variability of the different cell donors. On the other hand, the use of cell pools offers an opportunity to use less donor tissue and to perform long-term projects with a broad spectrum of analysis and replications. In the literature, information about the donors of cell pools, the procedure used for pooling, and the characterization/validation of cell pools is often lacking. In this study, we established three cell pools consisting of M. rhomboideus or M. longissimus from ten or six piglets, each with one gender and medium birth weight. Real-time impedimetric monitoring was used to evaluate the proliferative growth behavior of myoblasts for the cell pools in comparison to their corresponding unpooled cells over a period of 72 h, with a measurement being taken every 30 min. For each of the tested cell pools, cell index, slope, and doubling time did not differ between the cell pool and the unpooled cells of the donor animals. Differentiation capacity and mRNA expression of PAX7, MYOD and MYOG remained unchanged between the cell pool and the unpooled cells. Current results support that the use of cell pools is an appropriate method to reflect the average proliferative growth behavior of unpooled cells.
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