ArticleNature biotechnology2020
Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular proteins.
Article in Nature biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
77 citing papers in PubMed, 148 citations in OpenAlex.
- Tunable population diversity through a noise-driven differentiation switch.bioRxiv : the preprint server for biology · 2026Article
- Single-cell mapping of homocysteine-induced perturbations in avian embryogenesis using LMO and BD rhapsody transcriptomics.iScience · 2026Article
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- Functional characterization of specialized immune cells in a cnidarian reveals an ancestral antiviral program.Nature communications · 2026Article
- Integrating and mapping single-cell transcriptomics across the entire gene expression space.Briefings in bioinformatics · 2026Article
- ApoE-Dependent Lipid Handling by Median Eminence Microglia Preserves Myelin Integrity and Metabolic Function.bioRxiv : the preprint server for biology · 2026Article
- fastdemux: Robust SNP-based demultiplexing of single-cell population genomics data.bioRxiv : the preprint server for biology · 2026Article
- The role of cohesin loading at enhancers in the flux of loop extrusion and long-range transcriptional control.bioRxiv : the preprint server for biology · 2026Article
- Methodologies for Sample Multiplexing and Computational Deconvolution in Single-Cell Sequencing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
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- Antibody-oligonucleotide conjugates for spatial proteomics: principles, applications, and challenges.Acta biochimica et biophysica Sinica · 2025Article
- Empowering low-crosstalk, dynamic-decision random access of DNA storage via 384-multiplexed nanopore signatures.Nature communications · 2025Article
- PerturbNet predicts single-cell responses to unseen chemical and genetic perturbations.Molecular systems biology · 2025Article
- Probability of stealth multiplets in sample-multiplexing for droplet-based single-cell analysis.BMC genomics · 2025Article
- Article
- Advances and applications in single-cell and spatial genomics.Science China. Life sciences · 2025Review
- Active repression of cell fate plasticity by PROX1 safeguards hepatocyte identity and prevents liver tumorigenesis.Nature genetics · 2025Article
- Multiplexing cortical brain organoids for the longitudinal dissection of developmental traits at single-cell resolution.Nature methods · 2025Article
- Systematic benchmark of single-cell hashtag demultiplexing approaches reveals robust performance of a clustering-based method.Briefings in functional genomics · 2025Article
- inDrops-2: a flexible, versatile and cost-efficient droplet microfluidic approach for high-throughput scRNA-seq of fresh and preserved clinical samples.Nucleic acids research · 2025Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a universal sample multiplexing method for single-cell RNA sequencing in which fixed cells are chemically labeled by attaching identifying DNA oligonucleotides to cellular proteins. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional states that cannot be discerned from bulk measurements, establishing an efficient method for surveying cell populations from large experiments or clinical samples with the depth and resolution of single-cell RNA sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.