ArticleScientific reports2019
Detection and functional characterization of sigma class GST in Phlebotomus argentipes and its role in stress tolerance and DDT resistance.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 38 citations in OpenAlex.
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- Transgenerational effects of peppermint essential oil on life table parameters and antioxidant enzymes in Callosobruchus maculatus.Scientific reports · 2025Article
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- Glutathione S-transferase TcGSTu1 contributes to defense against eucalyptol in Tribolium castaneum.Genetica · 2024Article
- Comparative Genomics Uncovers the Evolutionary Dynamics of Detoxification and Insecticide Target Genes Across 11 Phlebotomine Sand Flies.Genome biology and evolution · 2024Article
- Knockdown resistance mutations in Phlebotomus argentipes sand flies in Bihar, India.Parasites & vectors · 2024Article
- Sigma-Class Glutathione Transferases (GSTσ): A New Target with Potential for Helminth Control.Tropical medicine and infectious disease · 2024Review
- Identification and Functional Analysis of an Epsilon Class Glutathione S-Transferase Gene Associated with α-Pinene Adaptation inInternational journal of molecular sciences · 2023Article
- Identification of metabolizing enzyme genes associated with xenobiotics and odorants in the predatory stink bugHeliyon · 2023Article
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- Polyphagous insectHeliyon · 2022Article
- Characterization of the glutathione S-transferase genes in the sand flies Phlebotomus papatasi and Lutzomyia longipalpis shows expansion of the novel glutathione S-transferase xi (X) class.Insect molecular biology · 2022Article
- Biochemical characterization and peptide mass fingerprinting of two glutathione transferases from Biomphalaria alexandrina snails (Gastropoda: Planorbidae).Journal, genetic engineering & biotechnology · 2022Article
- Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies.Frontiers in physiology · 2022Review
- A full-length transcriptome and gene expression analysis of three detoxification gene families in a predatory stink bug,Frontiers in physiology · 2022Article
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- Transcriptome Sequencing of the Striped Cucumber Beetle,Biotech (Basel (Switzerland)) · 2020Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several Glutathione S-transferases (GSTs) enzymes, in insects, have previously been implicated in resistance developed against DDT and other insecticides. The GST enzyme particularly sigma class have important physiological role in detoxification of lipid peroxidation by-products in insects. Phlebotomus argentipes has been intensely exposed to DDT over years due to Indoor Residual Spray (IRS) programme for Kala-azar elimination in Bihar, India. However, in P. argentipes, role of GSTs in DDT resistance have not been elucidated. Here, sigma class GST of P. argentipes (Parg-GSTσ) was successfully cloned, expressed and purified by affinity chromatography. The recombinant Parg-GSTσ was found to be highly active towards cumene hydroperoxide and 4-HNE having specific activity 92.47 & 203.92 µM/min/mg of protein, respectively and exhibited low activity towards universal substrate CDNB i.e., 8.75 µM/min/mg of protein. RT-PCR and immunoblot analysis showed at least 2 and 1.8 fold overexpression of Parg-GSTσ in the single exposed and non exposed DDT resistant P. argentipes as compared to susceptible, implicating Parg-GSTσ also involved in DDT resistance probably by imparting enhanced stress tolerance. The DDT, H
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