ArticleScientific reports2019
Slow Freezing Versus Vitrification of Mouse Ovaries: from Ex Vivo Analyses to Successful Pregnancies after Auto-Transplantation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Protective Effect of Glutathione Supplementation On Mouse Ovarian Tissue Vitrification and Autologous Transplantation.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- BEZ235-Mediated PI3K/mTOR dual inhibition improves ovarian follicle survival in a preclinical model.Reproductive biology and endocrinology : RB&E · 2025Article
- Ovarian cryopreservation with rapamycin improves fertility restoration in a murine orthotopic transplantation model.Scientific reports · 2025Article
- Cryopreservation of human kidney organoids.Cellular and molecular life sciences : CMLS · 2024Article
- The mTOR Inhibitor Rapamycin Counteracts Follicle Activation Induced by Ovarian Cryopreservation in Murine Transplantation Models.Medicina (Kaunas, Lithuania) · 2023Article
- Comparison of angiogenic potential in vitrified vs. slow frozen human ovarian tissue.Scientific reports · 2023Article
- Impacts of vitrification on the transcriptome of human ovarian tissue in patients with gynecological cancer.Frontiers in genetics · 2023Article
- Evaluation of an alternative heterotopic transplantation model for ovarian tissue to test pharmaceuticals improvements for fertility restoration.Reproductive biology and endocrinology : RB&E · 2022Article
- Comparison between slow freezing and vitrification of ovarian tissue cryopreservation in assigned female at birth transgender people receiving testosterone therapy: data on histological and viability parameters.Journal of assisted reproduction and genetics · 2022Article
- Identification of Biomarkers for Predicting Ovarian Reserve of Primordial FollicleFrontiers in genetics · 2022Article
- The effect of different vitrification protocols on cell survival in human ovarian tissue: a pilot study.Journal of ovarian research · 2021Article
- Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture.Journal of ovarian research · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Slow freezing (SF) is the reference method for ovarian tissue cryopreservation. Vitrification (VT) constitutes an alternative but controversial method. This study compares SF and VT (open [VTo] and closed [VTc] systems) in terms of freezing damage and fertility restoration ability. In vitro analyses of C57Bl/6 SF or VTo-ovaries, immediately after thawing/warming or after culture (cult), revealed that event though follicular density was similar between all groups, nuclear density was decreased in VTo-ovaries compared to CT-ovaries (CT = 0.50 ± 0.012, SF = 0.41 ± 0.03 and VTo = 0.29 ± 0.044, p < 0.01). Apoptosis was higher in VTo-cult ovaries compared to SF-cult ovaries (p < 0.001) whereas follicular Bmp15 and Amh gene expression levels were decreased in the ovaries after culture, mostly after VTo (p < 0.001). Natural mating after auto-transplantation of SF, VTo and VTc-ovaries revealed that most mice recovered their oestrous cycle. Fertility was only restored with SF and VTo ovaries (SF: 68%; VTo: 63%; VTc: 0%; p < 0.001). Mice auto-transplanted with SF and VTo-ovaries achieved the highest number of pregnancies. In conclusion, in vitro, no differences between SF and VTo were evident immediately after thawing/warming but VTo ovaries displayed alterations in apoptosis and follicular specific proteins after culture. In vivo, SF and VTo ovary auto-transplantation fully restored fertility whereas with VTc-ovary auto-transplantation no pregnancies were achieved.
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