Evidence map›Paper›PMID 31873116›Full record

ArticleScientific reports2019

Modulation of Wnt/β-catenin signaling promotes blood-brain barrier phenotype in cultured brain endothelial cells.

Marlyn D Laksitorini, Vinith Yathindranath, Wei Xiong, Sabine Hombach-Klonisch, Donald W Miller

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
72citing papers in PubMed, 2 pooled it
4.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

72 citing papers in PubMed, 2 syntheses or guidelines pooled it, 101 citations in OpenAlex.

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12 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Marlyn D LaksitoriniDepartment of Pharmacology and Theurapetics, Max Rady College of Medicine, University of Manitoba, Winnipeg, R3E 0T6, Canada.
Vinith YathindranathDepartment of Pharmacology and Theurapetics, Max Rady College of Medicine, University of Manitoba, Winnipeg, R3E 0T6, Canada.
Wei XiongDepartment of Pharmacology and Theurapetics, Max Rady College of Medicine, University of Manitoba, Winnipeg, R3E 0T6, Canada.
Sabine Hombach-KlonischDepartment of Human Anatomy and Cell Science, Max Rady College of Medicine, University of Manitoba, Winnipeg, R3E 0J9, Canada.
Donald W MillerDepartment of Pharmacology and Theurapetics, Max Rady College of Medicine, University of Manitoba, Winnipeg, R3E 0T6, Canada. Donald.Miller@umanitoba.ca.
University of Manitoba · CAHealth Sciences Centre · CA

Funding

CIHR
6 · The paper itself

Abstract

Wnt/β-catenin signaling is important for blood-brain barrier (BBB) development and is implicated in BBB breakdown under various pathophysiological conditions. In the present study, a comprehensive characterization of the relevant genes, transport and permeability processes influenced by both the autocrine and external activation of Wnt signaling in human brain endothelial cells was examined using hCMEC/D3 culture model. The hCMEC/D3 expressed a full complement of Wnt ligands and receptors. Preventing Wnt ligand release from hCMEC/D3 produced minimal changes in brain endothelial function, while inhibition of intrinsic/autocrine Wnt/β-catenin activity through blocking β-catenin binding to Wnt transcription factor caused more modest changes. In contrast, activation of Wnt signaling using exogenous Wnt ligand (Wnt3a) or LiCl (GSK3 inhibitor) improved the BBB phenotypes of the hCMEC/D3 culture model, resulting in reduced paracellular permeability, and increased P-glycoprotein (P-gp) and breast cancer resistance associated protein (BCRP) efflux transporter activity. Further, Wnt3a reduced plasmalemma vesicle associated protein (PLVAP) and vesicular transport activity in hCMEC/D3. Our data suggest that this in vitro model of the BBB has a more robust response to exogenous activation of Wnt/β-catenin signaling compared to autocrine activation, suggesting that BBB regulation may be more dependent on external activation of Wnt signaling within the brain microvasculature.

Indexed as

Wnt Signaling PathwayAutocrine CommunicationBlood-Brain BarrierCells, CulturedEndocytosisEndothelial CellsGene Expression RegulationHumansLigandsLithium ChlorideMembrane ProteinsOxazolesPhenotypeReceptors, Cell SurfaceiCRT3 compoundLigandsLithium ChlorideMembrane ProteinsOxazolesPLVAP protein, humanReceptors, Cell Surface

Identifiers

PMID31873116
PMCPMC6928218
OpenAlexW2997752650

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.