ArticleNucleic acids research2020
Widespread roles for piRNAs and WAGO-class siRNAs in shaping the germline transcriptome of Caenorhabditis elegans.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 83 citations in OpenAlex.
- Systematic identification of germ granule proteins reveals specialized roles in RNAi and small RNA inheritance.Genes & development · 2026Article
- DivergenteLife · 2026Article
- A TCER-1-siRNA regulatory axis suppresses antibacterial innate immunity in C. elegans.PLoS pathogens · 2026Article
- Systematic Identification of Germ Granule Proteins Reveals Specialized Roles in RNAi and Small RNA Inheritance.bioRxiv : the preprint server for biology · 2026Article
- RG motifs promote piRNA-mediated gene silencing in C. elegans.Nucleic acids research · 2025Article
- Remodeling of Germ Cell mRNPs for Translational Control.Biology · 2025Review
- WAGO-1 is a sexually dimorphic Argonaute protein required for proper germ granule structure and gametogenesis.bioRxiv : the preprint server for biology · 2025Article
- FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline.Development (Cambridge, England) · 2025Article
- Article
- Piwi mutant germ cells transmit a form of heritable stress that promotes longevity.Aging cell · 2025Article
- Recent progress in regulation of aging by non-coding RNAs inAnimal cells and systems · 2025Review
- A low-abundance class of Dicer-dependent siRNAs produced from a variety of features inGenome research · 2024Article
- HERD-1 mediates multiphase condensate immiscibility to regulate small RNA-driven transgenerational epigenetic inheritance.Nature cell biology · 2024Article
- Pachytene piRNAs control discrete meiotic events during spermatogenesis and restrict gene expression in space and time.Science advances · 2024Article
- Small RNA-mediated genetic switches coordinate ALG-3/4 small RNA pathway function.Nucleic acids research · 2024Article
- Article
- Heritable epigenetic changes are constrained by the dynamics of regulatory architectures.eLife · 2024Article
- Genome-wide association and environmental suppression of the mortal germline phenotype of wild C. elegans.EMBO reports · 2023Article
- C. elegans germ granules sculpt both germline and somatic RNAome.Nature communications · 2023Article
- Sensitized piRNA reporter identifies multiple RNA processing factors involved in piRNA-mediated gene silencing.Genetics · 2023Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Piwi-interacting RNAs (piRNAs) and small interfering RNAs (siRNAs) are distinct classes of small RNAs required for proper germline development. To identify the roles of piRNAs and siRNAs in regulating gene expression in Caenorhabditis elegans, we subjected small RNAs and mRNAs from the gonads of piRNA and siRNA defective mutants to high-throughput sequencing. We show that piRNAs and an abundant class of siRNAs known as WAGO-class 22G-RNAs are required for proper expression of spermatogenic and oogenic genes. WAGO-class 22G-RNAs are also broadly required for transposon silencing, whereas piRNAs are largely dispensable. piRNAs, however, have a critical role in controlling histone gene expression. In the absence of piRNAs, histone mRNAs are misrouted into the nuclear RNAi pathway involving the Argonaute HRDE-1, concurrent with a reduction in the expression of many histone mRNAs. We also show that high-level gene expression in the germline is correlated with high level 22G-RNA production. However, most highly expressed genes produce 22G-RNAs through a distinct pathway that presumably involves the Argonaute CSR-1. In contrast, genes targeted by the WAGO branch of the 22G-RNA pathway are typically poorly expressed and respond unpredictably to loss of 22G-RNAs. Our results point to broad roles for piRNAs and siRNAs in controlling gene expression in the C. elegans germline.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.