Evidence map›Paper›PMID 31872200›Full record

ArticleLab on a chip2020

Extended culture and imaging of normal and regenerating adult zebrafish hearts in a fluidic device.

Joycelyn K Yip, Michael Harrison, Jessi Villafuerte, G Esteban Fernandez, Andrew P Petersen, Ching-Ling Lien, Megan L McCain

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lab on a chip, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Modelling human brain development and disease with organoids.Nature reviews. Molecular cell biology · 2025
    Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Visualization of regenerating and repairing hearts.Clinical science (London, England : 1979) · 2022
    Article
  10. Article
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Joycelyn K YipLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu.
Michael HarrisonHeart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA. clien@chla.usc.edu and The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Jessi VillafuerteHeart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA. clien@chla.usc.edu and The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA and Department of Biology, California State University of San Bernardino, San Bernardino, CA 92407, USA.
G Esteban FernandezThe Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Andrew P PetersenLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu.
Ching-Ling LienHeart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA. clien@chla.usc.edu and The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA and Department of Surgery, Keck School of Medicine of USC, University of Southern California, Los Angeles, CA 90033, USA and Department of Biochemistry and Molecular Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, CA 90033, USA.
Megan L McCainLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu and Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, CA 90033, USA.ORCID 0000-0003-1908-6783
University of Southern California · USChildren's Hospital of Los Angeles · US

Funding

Cxc12 chemokine signaling regulates synchronous development of coronary vessels and myocardiumR01HL130172 · NHLBI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI LIEN, CHING-LING E · 2017 to 2020
$1.7M
American Heart Association-American Stroke Association 16SDG29950005NHLBI NIH HHS R01 HL130172
6 · The paper itself

Abstract

Myocardial infarction and heart failure are leading causes of death worldwide, in large part because adult human myocardium has extremely limited regeneration capacity. Zebrafish are a powerful model for identifying new strategies for human cardiac repair because their hearts regenerate after relatively severe injuries. Zebrafish are also relatively scalable and compatible with many genetic tools. However, characterizing the regeneration process in live adult zebrafish hearts has proved challenging because adult fish are opaque, preventing live imaging in vivo. An alternative strategy is to explant and culture intact adult zebrafish hearts and investigate them ex vivo. However, explanted hearts maintained in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we designed and fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. We then compared the morphology and calcium activity of hearts cultured in the device, hearts cultured statically in dishes, and freshly explanted hearts. After one week of culture, hearts in the device experienced significantly less morphological degradation compared to hearts cultured in dishes. Hearts cultured in devices for one week also maintained capture rates similar to fresh hearts, unlike hearts cultured in dishes. We then cultured explanted injured hearts in the device and used live imaging techniques to continuously record the myocardial revascularization process over several days, demonstrating how our device is compatible with long-term live imaging and thereby enables unprecedented visual access to the multi-day process of adult zebrafish heart regeneration.

Indexed as

Lab-On-A-Chip DevicesTissue Culture TechniquesAnimalsHeartZebrafish

Identifiers

PMID31872200
PMCPMC8015799
OpenAlexW2996197320

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.