ReviewAngewandte Chemie (International ed. in English)2020
Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays.
Review in Angewandte Chemie (International ed. in English), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Real-Time Digital Micromotor Tracking-Enabled Ultrasensitive Immunoassay.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Peptide Aptamer-Enabled Nanoplasmonic Digital Immunoassay for Ultrasensitive Cytokine Sensing in Early Inflammation and Immune Modulation.ACS sensors · 2026Article
- Single-molecule readout of reversible nanoswitches enables continuous monitoring of low biomarker concentrations.Nature communications · 2026Article
- Breaking the Low Concentration Barrier of Single-Molecule Fluorescence Quantification to the Sub-Picomolar Range.Small methods · 2026Article
- Robust Development of Gold Nanorod-Quantum Dot Assemblies for Dynamic Dual-Modal Single Nanoparticle Imaging and Tracking Applications.ACS applied nano materials · 2025Article
- Digital Immunoassay for Biomarker Detection Based on Single-Particle Laser Ablation ICP MS.Analytical chemistry · 2025Article
- Single-Molecule Detection of Optical Signals Using DNA-Based Plasmonic Nanostructures.Biosensors · 2025Review
- Partition-Less Digital Immunoassay Using Configurable Topographic Nanoarrays for Extracellular Vesicle Diagnosis of Ewing Sarcoma.ACS nano · 2025Article
- How Highly Heterogeneous Sensors with Single-Molecule Resolution can Result in Robust Continuous Monitoring Over Long Time Spans.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Deep learning-assisted single-atom detection of copper ions by combining click chemistry and fast scan voltammetry.Nature communications · 2024Article
- Upconversion Nanoparticle-Based Dot-Blot Immunoassay for Quantitative Biomarker Detection.Analytical chemistry · 2024Article
- Sandwich Immuno-RCA Assay with Single Molecule Counting Readout: The Importance of Biointerface Design.ACS applied materials & interfaces · 2024Article
- Neural network informed photon filtering reduces fluorescence correlation spectroscopy artifacts.Biophysical journal · 2024Article
- Ultrasensitive, Multiplexed Buoyant Sensor for Monitoring Cytokines in Biofluids.Nano letters · 2023Article
- Post-Assay Chemical Enhancement for Highly Sensitive Lateral Flow Immunoassays: A Critical Review.Biosensors · 2023Review
- High-Throughput Single-Molecule Sensors: How Can the Signals Be Analyzed in Real Time for Achieving Real-Time Continuous Biosensing?ACS sensors · 2023Article
- Digital and Absolute Assays for Low Abundance Molecular Biomarkers.Accounts of chemical research · 2023Article
- Single-Molecule Optical Biosensing: Recent Advances and Future Challenges.ACS physical chemistry Au · 2023Review
- Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay.Analytical chemistry · 2023Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to detect low concentrations of analytes and in particular low-abundance biomarkers is of fundamental importance, e.g., for early-stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single-molecule bioaffinity assays. While many review articles have highlighted the potentials of single-molecule technologies for analytical and diagnostic applications, these technologies are not as widespread in real-world applications as one should expect. This Review provides a theoretical background on single-molecule-or better digital-assays to critically assess their potential compared to traditional analog assays. Selected examples from the literature include bioaffinity assays for the detection of biomolecules such as proteins, nucleic acids, and viruses. The structure of the Review highlights the versatility of optical single-molecule labeling techniques, including enzymatic amplification, molecular labels, and innovative nanomaterials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.