Evidence map›Paper›PMID 31869197›Full record

ArticleAnalytical chemistry2020

Automated Spatially Targeted Optical Microproteomics (autoSTOMP) to Determine Protein Complexity of Subcellular Structures.

Bocheng Yin, Roberto Mendez, Xiao-Yu Zhao, Rishi Rakhit, Ku-Lung Hsu, Sarah E Ewald

Open access · greenAbstract read
In one paragraph

Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Cysteine-S-nitrosylation inhibits Rop5-mediated immune evasion inbioRxiv : the preprint server for biology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Bocheng YinDepartment of Microbiology, Immunology and Cancer Biology and the Carter Immunology Center , University of Virginia School of Medicine , Charlottesville , Virginia 22908-0395 , United States.ORCID 0000-0003-1755-2728
Roberto MendezDepartment of Chemistry , University of Virginia , Charlottesville , Virginia 22904-4132 , United States.ORCID 0000-0001-5355-112X
Xiao-Yu ZhaoDepartment of Microbiology, Immunology and Cancer Biology and the Carter Immunology Center , University of Virginia School of Medicine , Charlottesville , Virginia 22908-0395 , United States.ORCID 0000-0001-5095-7212
Rishi RakhitMitokinin Inc , 953 Indiana Street , San Francisco , California 94107-3007 , United States.
Ku-Lung HsuDepartment of Chemistry , University of Virginia , Charlottesville , Virginia 22904-4132 , United States.ORCID 0000-0001-5620-3972
Sarah E EwaldDepartment of Microbiology, Immunology and Cancer Biology and the Carter Immunology Center , University of Virginia School of Medicine , Charlottesville , Virginia 22908-0395 , United States.ORCID 0000-0002-5327-7578
University of Virginia · USMitokinin (United States) · US

Funding

Innate Inflammatory Control of CachexiaR35GM138381 · NIGMS · UNIVERSITY OF VIRGINIA · PI Sarah E. Ewald · 2020 to 2026
$2.9M
Proteomic interrogation of the parasite vacuole using autoSTOMPR21AI156153 · NIAID · UNIVERSITY OF VIRGINIA · PI EWALD, SARAH E. · 2021 to 2022
$444k
Identification of the non-proteolytic mechanism of NLRP1 activationK22AI116727 · NIAID · UNIVERSITY OF VIRGINIA · PI EWALD, SARAH E. · 2016 to 2017
$269k
NIAID NIH HHS K22 AI116727NIAID NIH HHS R21 AI156153NIGMS NIH HHS R35 GM138381
6 · The paper itself

Abstract

Spatially targeted optical microproteomics (STOMP) is a method to study region-specific protein complexity in primary cells and tissue samples. STOMP uses a confocal microscope to visualize structures of interest and to tag the proteins within those structures by a photodriven cross-linking reaction so that they can be affinity purified and identified by mass spectrometry (

Indexed as

AutomationProteomicsAnimalsDendritic CellsFibroblastsHumansMiceMolecular StructureOptical ImagingProtein ConformationProteinsProteins

Identifiers

PMID31869197
PMCPMC8454273
OpenAlexW2998584215

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.