ArticleProceedings of the National Academy of Sciences of the United States of America1988
A 22-nucleotide spliced leader sequence in the human parasitic nematode Brugia malayi is identical to the trans-spliced leader exon in Caenorhabditis elegans.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 68 citations in OpenAlex.
- SLRanger: an integrated approach for spliced leader detection and operon prediction using long RNA reads.Briefings in bioinformatics · 2025Article
- Heterodera glycines utilizes promiscuous spliced leaders and demonstrates a unique preference for a species-specific spliced leader over C. elegans SL1.Scientific reports · 2019Article
- Prolyl 4-hydroxlase activity is essential for development and cuticle formation in the human infective parasitic nematode Brugia malayi.The Journal of biological chemistry · 2013Article
- Dynamic regulation of alternative splicing by silencers that modulate 5' splice site competition.Cell · 2008Article
- Sequences necessary for trans-splicing in transiently transfected Brugia malayi.Molecular and biochemical parasitology · 2007Article
- Analysis and functional classification of transcripts from the nematode Meloidogyne incognita.Genome biology · 2003Article
- Orphon spliced-leader sequences form part of a repetitive element in Angiostrongylus cantonensis.Nucleic acids research · 1995Article
- Functional analysis of cis-acting DNA elements required for expression of the SL RNA gene in the parasitic protozoan Leishmania amazonensis.Nucleic acids research · 1994Article
- In Euglena, spliced-leader RNA (SL-RNA) and 5S rRNA genes are tandemly repeated.Nucleic acids research · 1992Article
- Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Spliced leader RNA and 5S rRNA genes in Herpetomonas spp. are genetically linked.Nucleic acids research · 1992Article
- Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12.Molecular & general genetics : MGG · 1991Article
- Insertion of part of an intron into the 5' untranslated region of a Caenorhabditis elegans gene converts it into a trans-spliced gene.Molecular and cellular biology · 1991Article
- mRNA processing in the Trypanosomatidae.Experientia · 1991Review
- A spliced leader is present on a subset of mRNAs from the human parasite Schistosoma mansoni.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Many transcribed regions of the Onchocerca volvulus genome contain the spliced leader sequence of Caenorhabditis elegans.Molecular and cellular biology · 1990Article
- Transcription of a nematode trans-spliced leader RNA requires internal elements for both initiation and 3' end-formation.The EMBO journal · 1990Article
- Transcription and cap trimethylation of a nematode spliced leader RNA in a cell-free system.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Two highly conserved transcribed regions in the 5S DNA repeats of the nematodes Caenorhabditis elegans and Caenorhabditis briggsae.Nucleic acids research · 1989Article
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5 authors at 2 institutions in 1 country.
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Abstract
The mRNAs encoding a 63-kDa antigen in the human parasitic nematode Brugia Malayi contain a spliced leader sequence of 22 nucleotides (nt) that is identical to the trans-spliced leader found on certain actin mRNAs in the distantly related nematode Caenorhabditis elegans. The 22-nt sequence does not appear to be encoded near the 63-kDa genes but is present in multiple copies in several locations within the parasite genome, including the 5S rRNA gene repeat. The 5S-linked copies of the 22-nt sequence are transcribed to yield a 109-nt nonpolyadenylated RNA with the 22-nt leader sequence at its 5' end. We suggest that the 22-nt leader is acquired by 63-kDa antigen mRNAs through trans-splicing. These results indicate that trans-splicing is widespread in nematodes and argue for the functional significance of the 22-nt spliced leader exon in nematode mRNA metabolism.
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