ArticleThe Journal of veterinary medical science2020
Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases.
Article in The Journal of veterinary medical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Integrated pathological and molecular characterisation of respiratory disease in extensively reared Sicilian Black pigs: detection of Mycoplasma hyopneumoniae, porcine circovirus type 2, and concurrent pulmonary silicosis.Veterinary research communications · 2026Article
- FBXO5 drives hepatocellular carcinoma progression and is a target for tea polyphenol-mediated inhibition.Translational cancer research · 2025Article
- Advances in molecular epidemiology and detection methods of pseudorabies virus.Discover nano · 2025Review
- Multiplex droplet digital PCR for the detection and quantitation ofFrontiers in cellular and infection microbiology · 2025Article
- Health-Economic Impact Attributable to Occurrence of Pleurisy and Pneumonia Lesions in Finishing Pigs.Veterinary sciences · 2024Article
- Exploring the Genetic Diversity ofMicroorganisms · 2024Article
- A marker-free genetic manipulation method for Glaesserella parasuis strains developed by alternately culturing transformants at 37°C and 30°C.BMC biotechnology · 2024Article
- Transcriptome sequencing reveals non-coding RNAs respond to porcine reproductive and respiratory syndrome virus andJournal of animal science and technology · 2024Article
- Prevalence and Genetic Characterization of Porcine Respiratory Coronavirus in Korean Pig Farms.Animals : an open access journal from MDPI · 2024Article
- Assessment of the potential occurrence ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Simultaneous Detection of Porcine Respiratory Coronavirus, Porcine Reproductive and Respiratory Syndrome Virus, Swine Influenza Virus, and Pseudorabies Virus via Quadruplex One-Step RT-qPCR.Pathogens (Basel, Switzerland) · 2024Article
- Article
- RPA-CRISPR/Cas12a-Based Detection ofAnimals : an open access journal from MDPI · 2023Article
- Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR.Microbiology spectrum · 2023Article
- Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019-2021.Veterinary sciences · 2023Article
- Development of a new multiplex quantitative PCR for the detection of Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae.MicrobiologyOpen · 2023Article
- Associations between Pleurisy and the Main Bacterial Pathogens of the Porcine Respiratory Diseases Complex (PRDC).Animals : an open access journal from MDPI · 2023Article
- Development of a one-run real-time PCR detection system for pathogens associated with poultry infectious diseases.The Journal of veterinary medical science · 2023Article
- A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs.Veterinary research · 2023Article
- Porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus alone or associated are frequent intralesional detected viruses in porcine respiratory disease complex cases in Northern Italy.Frontiers in veterinary science · 2023Article
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The etiology of Porcine respiratory disease complex is complicated by infections with multiple pathogens, and multiple infections increase the difficulty in identifying the causal pathogen. In this present study, we developed a detection system of microbes from porcine respiratory by using TaqMan real-time PCR (referred to as Dempo-PCR) to screen a broad range of pathogens associated with porcine respiratory diseases in a single run. We selected 17 porcine respiratory pathogens (Actinobacillus pleuropneumoniae, Boldetella bronchiseptica, Haemophilus parasuis, Pasteurella multocida, Pasteurella multocida toxin, Streptococcus suis, Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycoplasma hyosynovie, porcine circovirus 2, pseudorabies virus, porcine cytomegalovirus, swine influenza A virus, porcine reproductive and respiratory virus US strain, EU strain, porcine respiratory coronavirus and porcine hemagglutinating encephalomyelitis virus) as detection targets and designed novel specific primer-probe sets for seven of them. In sensitivity test by using standard curves from synthesized DNA, all primer-probe sets showed high sensitivity. However, porcine reproductive and respiratory virus is known to have a high frequency of genetic mutations, and the primer and probe sequences will need to be checked at a considerable frequency when performing Dempo-PCR from field samples. A total of 30 lung samples from swine showing respiratory symptoms on six farms were tested by the Dempo-PCR to validate the assay's clinical performance. As the results, 12 pathogens (5 virus and 7 bacteria) were detected and porcine reproductive and respiratory virus US strain, Mycoplasma hyorhinis, Haemophilus parasuis, and porcine cytomegalovirus were detected at high frequency. These results suggest that Dempo-PCR assay can be applied as a screening system with wide detection targets.
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