ArticleProtoplasma2020
Utilisation of Toluidine blue O pH 4.0 and histochemical inferences in plant sections obtained by free-hand.
Article in Protoplasma, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
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- Use of polyethylene glycol as an alternative to optimal cutting temperature medium in freeze sectioning for plant histochemical studies.Protoplasma · 2025Article
- Standardization of pharmacognostic characters and phytochemical study of Limeum obovatum vicary.Scientific reports · 2025Article
- Interaction of Capnodium alfenasii with extrafloral nectaries of Azadirachta indica.Protoplasma · 2025Article
- Combined Effects of Microgravity and Chronic Low-Dose Gamma Radiation onPlants (Basel, Switzerland) · 2024Article
- The Hemibiotrophic Apple Scab FungusJournal of fungi (Basel, Switzerland) · 2024Article
- Effects of Phloretin on Seedling Growth and Histochemical Distribution of Phenols, Polysaccharides and Lipids inPlants (Basel, Switzerland) · 2024Article
- Chemical and structural heterogeneity of olive leaves and their trichomes.Communications biology · 2024Article
- The Anthocyanin Accumulation RelatedInternational journal of molecular sciences · 2022Article
- Light and electron microscopies reveal unknown details of the pollen grain structure and physiology from Brazilian Cerrado species.Protoplasma · 2022Article
- An Evaluation of Traits, Nutritional, and Medicinal Component Quality ofFrontiers in plant science · 2022Article
- Ultrastructure of Terpene and Polyphenol Synthesis in the Bark ofFrontiers in microbiology · 2022Article
- Phenolic Constituents ofMolecules (Basel, Switzerland) · 2020Article
- Unravelling Chemical Composition of Agave Spines: News fromPlants (Basel, Switzerland) · 2020Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
For the observation of plant material under a light microscope, free-hand sectioning is usually the fastest and most practical procedure. The staining of the obtained cuts is a crucial step and the action mechanism of the dye is variable. Toluidine blue O is a cationic dye whose metachromatic potential for free-hand sections has long been known. Thus, this work aimed at analysing the performance of Toluidine blue O for this type of sectioning with a systematic and comparative approach. The action of Toluidine blue O pH 4.0 was tested for both fresh and previously clarified free-hand sections from leaves of different species of plant. These sections were compared with sections embedded in glycol-methacrylate resin stained with the same dye or submitted to different histochemical tests. The fresh sections obtained by free-hand and stained with Toluidine blue O permitted the accomplishment of histochemical inferences, with the advantage of maintaining the natural colours from the tissues. The previously clarified sections presented the advantage of having a clearer image, allowing a better cellular delimitation. We concluded that Toluidine blue O is very informative for sections of plant material obtained by free-hand, being, therefore, a procedure that is fast, inexpensive and with excellent results.
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