Evidence map›Paper›PMID 31863959›Full record

ArticleBone2020

Selective inhibition of progesterone receptor in osteochondral progenitor cells, but not in mature chondrocytes, modulated subchondral bone structures.

Chenlin Dai, Junjing Jia, Alexander Kot, Xueping Liu, Lixian Liu, Min Jiang, Nancy E Lane, Barton L Wise, Wei Yao

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bone, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Chenlin DaiCenter for Musculoskeletal Health, Department of Internal Medicine, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Junjing JiaCenter for Musculoskeletal Health, Department of Internal Medicine, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Alexander KotCenter for Musculoskeletal Health, Department of Internal Medicine, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Xueping LiuCenter for Musculoskeletal Health, Department of Internal Medicine, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Lixian LiuCenter for Musculoskeletal Health, Department of Internal Medicine, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Min JiangCenter for Musculoskeletal Health, Department of Internal Medicine, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Nancy E LaneCenter for Musculoskeletal Health, Department of Internal Medicine, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Barton L WiseCenter for Musculoskeletal Health, Department of Internal Medicine, University of California, Davis Medical Center, Sacramento, CA 95817, USA; Department of Orthopaedic Surgery, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Wei YaoCenter for Musculoskeletal Health, Department of Internal Medicine, University of California, Davis Medical Center, Sacramento, CA 95817, USA. Electronic address: yao@ucdavis.edu.
University of California Davis Medical Center · US

Funding

Building Interdisciplinary Research Careers in Womens Health at UC Davis - Administrative SupplementK12HD051958 · NICHD · UNIVERSITY OF CALIFORNIA DAVIS · PI LANE, NANCY E · 2005 to 2023
$9.6M
Sex Differences in response to an exercise for hyperkyphosisP50AR063043 · NIAMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI HAN, JAY J · 2012 to 2017
$5.7M
Building Interdisciplinary Research Careers in Women's Health at UC DavisK12AR084220 · NIAMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Nancy E Lane · 2023 to 2026
$3.0M
Directing mesenchymal stem cells to bone to augment bone formationR01AR061366 · NIAMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI YAO, WEI · 2012 to 2015
$1.1M
NIAMS NIH HHS K12 AR084220NIAMS NIH HHS P50 AR063043NIAMS NIH HHS R01 AR061366NICHD NIH HHS K12 HD051958
6 · The paper itself

Abstract

objectiveThe presence or relative proportion of progesterone nuclear receptors (PR) in different tissues may contribute to sexual dimorphism in these tissues. PR is expressed in chondrocytes, but its function is mostly unknown. We hypothesized that the PR may regulate chondrocyte metabolism and affect subchondral bone structure.

methodsWe utilized genetic fate mapping and immunohistochemistry to elucidate PR expression in and effect on cartilage. To define sex-dependent and chondrocyte-specific effects of the PR on subchondral bone, we selectively deleted PR in osteochondrogenic progenitor cells marked by Prx1 (Prx1; PRcKO) and Collagen 2 (Col2; PRcKO), or in matured chondrocytes marked by aggrecan (Acan; PRcKO) and evaluated subchondral bone structure at 4 months of age. Chondrocyte aging was monitored by anti-senescence marker p16INK4a, and MMP13, one of the Senescence-Associated Secretary Phenotype (SASP) components.

resultsCompared to wild-type (WT) mice, the female Prx1; PRcKO and the Col2; PRcKO mice had greater total subchondral bone volume and greater subchondral cortical bone thickness, with increased estimated subchondral bone stiffness and failure load in both female and male Col2; PRcKO mice. Moreover, Col2; PRcKO mice from both sexes had greater bone formation and bone strength at the femurs. In contrast, we did not observe any subchondral bone changes in Acan; PRcKO mice other than higher work-to-failure observed in the male Acan; PRcKO mice. Despite no detected difference in articular cartilage between the WT and the PR; chondrocyte conditional deletion mice, there were greater numbers of senescent chondrocytes and increased MMP13 expression, especially in the male mutant mice.

conclusionThese findings suggest that selective inhibition of PR in osteoprogenitor cells, but not in terminally differentiated chondrocytes, induced an increased subchondral bone phenotype and high estimated subchondral bone strength, which might be associated with the development of osteoarthritis in older age.

Indexed as

Cartilage, ArticularOsteoarthritisAnimalsChondrocytesFemaleMaleMiceReceptors, ProgesteroneStem CellsReceptors, ProgesteroneChondrocyteOsteoprogenitor cellsProgesterone receptorSelective knockoutSubchondral bone

Identifiers

PMID31863959
PMCPMC7006606
OpenAlexW2994890630

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.