Evidence map›Paper›PMID 31863411›Full record

ArticleMolecular biotechnology2020

Molecular Cloning and Expression of a Cryptochrome Gene CiCRY-DASH1 from the Antarctic microalga Chlamydomonas sp. ICE-L.

Xin Zhang, Zhou Zheng, Yingying He, Lina Liu, Changfeng Qu, Jinlai Miao

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Antarctic MicroalgaMarine drugs · 2026
    Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Xin ZhangSchool of Basic Medicine, Qingdao University, Qingdao, 266071, China.
Zhou ZhengFirst Institute of Oceanography, Ministry of Natural Resource, Qingdao, 266061, China.
Yingying HeSchool of Basic Medicine, Qingdao University, Qingdao, 266071, China.
Lina LiuSchool of Basic Medicine, Qingdao University, Qingdao, 266071, China.
Changfeng QuFirst Institute of Oceanography, Ministry of Natural Resource, Qingdao, 266061, China.
Jinlai MiaoFirst Institute of Oceanography, Ministry of Natural Resource, Qingdao, 266061, China. miaojinlai@fio.org.cn.ORCID http://orcid.org/0000-0002-6387-4229
Qingdao National Laboratory for Marine Science and Technology · CNQingdao University · CN

Funding

Basic Scientific Fund for National Public Research Institutes of China 2016Q10Key Research and Development Program of Shandong Province 2017GHY15112Key Research and Development Program of Shandong Province 2018GHY115039Key Research and Development Program of Shandong Province 2018YYSP024National Programme on Global Change and Air-sea Interaction No. GASI-03-02-02-05Ningbo Public Service Platform for High-Value Utilization of Marine Biological Resources NBHY-2017-P2the China Ocean Mineral Resources R&D Association No. DY135-B2-14the National Key Research and Development Program of China 2018YFD0900705the National Key Research and Development Program of China 2018YFD0901103the Natural Science Foundation of China 41576187the Natural Science Foundation of China 41776203the Natural Science Foundation of Shandong No. ZR2019BD023
6 · The paper itself

Abstract

Cryptochromes (CRYs) are flavin-binding proteins that sense blue and near-ultraviolet light and participate in the photoreactions of organisms and the regulation of biological clocks. In this study, the complete open reading frame (ORF) of CiCRY-DASH1 (GenBank ID MK392361), encoding one kind of cryptochrome, was cloned from the Antarctic microalga Chlamydomonas sp. ICE-L. The quantitative real-time PCR study showed that the CiCRY-DASH1 had the highest expression at 5 °C and salinity of 32‰. The CiCRY-DASH1 was positively regulated by blue, yellow, or red light. Moreover, the CiCRY-DASH1 can positively respond to extreme polar day and night treatment and exhibit a certain circadian rhythm, which indicated that CiCRY-DASH1 participated in the circadian clock and its expression was regulated by circadian rhythms. And the CiCRY-DASH1 was more noticeably affected by ultraviolet-B radiation than ultraviolet-A radiation, indicating ultraviolet-B light does obvious damage to Antarctic microalgae.

Indexed as

Amino Acid SequenceAntarctic RegionsChlamydomonasChlorophyllCircadian RhythmCloning, MolecularComputational BiologyCryptochromesDNA, ComplementaryGene ExpressionLightMicroalgaeOpen Reading FramesPhylogenySalinitySequence Homology, Amino AcidChlorophyllCryptochromesDNA, ComplementaryBioinformatic analysisChlorophyll fluorescenceExpression analysisLightQuantitative real-time PCR

Identifiers

PMID31863411
OpenAlexW2994982955

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.