ArticleScientific reports2019
Distinct metabolic profiles in Drosophila sperm and somatic tissues revealed by two-photon NAD(P)H and FAD autofluorescence lifetime imaging.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- A fluorescence lifetime separation approach for FLIM live-cell imaging.Journal of microscopy · 2026Article
- Gatekeepers of the Germ Line: How Mitochondria Shape Reproductive Evolution in Metazoans.Biology · 2025Review
- Label-free metabolic fingerprinting of motile mammalian spermatozoa with subcellular resolution.BMC biology · 2025Article
- Novel application of metabolic imaging of early embryos using a light-sheet on-a-chip device: a proof-of-concept study.Human reproduction (Oxford, England) · 2025Article
- Assessing the role of mitonuclear interactions on mitochondrial function and organismal fitness in naturalEvolution letters · 2024Article
- Drosophila melanogaster sperm turn more oxidative in the female.The Journal of experimental biology · 2024Article
- Label-free multimodal imaging of infected Galleria mellonella larvae.Scientific reports · 2022Article
- Imaging and spectral analysis of autofluorescence patterns in larval head structures of mosquito vectors.European journal of histochemistry : EJH · 2022Article
- Autofluorescent Biomolecules in Diptera: From Structure to Metabolism and Behavior.Molecules (Basel, Switzerland) · 2022Review
- Article
- Luminescence lifetime imaging of three-dimensional biological objects.Journal of cell science · 2021Article
- Somatic production of reactive oxygen species does not predict its production in sperm cells across Drosophila melanogaster lines.BMC research notes · 2021Article
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2 authors at 1 institution in 1 country.
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Abstract
Metabolic profiles vary across all levels of biological diversity, from cells to taxa. Two-photon fluorescence lifetime imaging microscopy (FLIM) facilitates metabolic characterisation of biological specimens by assaying the intrinsic autofluorescence of the ubiquitous coenzymes NAD(P)H and FAD. The potential of this method for characterising the diversity of organismal metabolism remains largely untapped. Using FLIM in Drosophila melanogaster, we show tissue-specificity in fluorescence lifetime that reflects variation in redox patterns. In particular, sperm cells exhibited elevated glycolysis relative to other tissues. We also show that sperm metabolism is phenotypically plastic: compared to male-stored sperm, sperm stored in the female's storage organ showed a substantial reduction in the protein-bound FAD lifetime fraction but no change in the NAD(P)H profile. This study represents the first ex vivo investigation of sperm metabolism using FLIM.
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