Evidence map›Paper›PMID 31862408›Full record

ArticleCancer letters2020

HOTAIRM1 lncRNA is downregulated in clear cell renal cell carcinoma and inhibits the hypoxia pathway.

Michael J Hamilton, Matthew Young, Kay Jang, Silvia Sauer, Vanessa E Neang, Alexia T King, Thomas Girke, Ernest Martinez

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 87 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Exosome-encapsulated lncRNA HOTAIRM1 contributes to PMScience China. Life sciences · 2024
    Article
  7. Review
  8. The influence of sex hormones on renal cell carcinoma.Therapeutic advances in medical oncology · 2024
    Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Michael J HamiltonDepartment of Biochemistry, University of California, Riverside, CA, USA.
Matthew YoungDepartment of Biochemistry, University of California, Riverside, CA, USA.
Kay JangDepartment of Biochemistry, University of California, Riverside, CA, USA.
Silvia SauerDepartment of Biochemistry, University of California, Riverside, CA, USA.
Vanessa E NeangDepartment of Biochemistry, University of California, Riverside, CA, USA.
Alexia T KingDepartment of Biochemistry, University of California, Riverside, CA, USA.
Thomas GirkeDepartment of Botany and Plant Sciences, University of California, Riverside, CA, USA.
Ernest MartinezDepartment of Biochemistry, University of California, Riverside, CA, USA. Electronic address: ernest.martinez@ucr.edu.
University of California, Riverside · US

Funding

UC Riverside MARC U* Star Undergraduate Research ProgramT34GM062756 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI MARTINEZ, ERNEST · 2001 to 2022
$8.7M
Functional characterization of the long non-coding RNA Scirocco in development anR01CA158540 · NCI · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI MARTINEZ, ERNEST · 2012 to 2017
$1.5M
Acquisition of a Scalable Storage Cluster for Data Intensive NIH ResearchS10OD016290 · OD · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI GIRKE, THOMAS · 2014 to 2014
$593k
NCI NIH HHS R01 CA158540NIGMS NIH HHS T34 GM062756
6 · The paper itself

Abstract

HOXA Transcript Antisense RNA, Myeloid-Specific 1 (HOTAIRM1) is a conserved long non-coding RNA (lncRNA) involved in myeloid and neural differentiation that is deregulated in acute myeloid leukemia and other cancers. Previous studies focused on the nuclear unspliced HOTAIRM1 transcript, however cytoplasmic splice variants exist whose roles have remained unknown. Here, we report novel functions of HOTAIRM1 in the kidney. HOTAIRM1 transcripts are induced during renal lineage differentiation of embryonic stem cells and required for expression of specific renal differentiation genes. We show that the major HOTAIRM1 transcript in differentiated cells is the spliced cytoplasmic HM1-3 isoform and that HM1-3 is downregulated in >90% of clear cell renal cell carcinomas (ccRCCs). Knockdown of HM1-3 in renal cells deregulates hypoxia-responsive and angiogenic genes, including ANGPTL4. Furthermore, HOTAIRM1 transcripts are downregulated by hypoxia-mimetic stress and knockdown of the cytoplasmic HM1-3 isoform in normoxic cells post-transcriptionally induces Hypoxia-Inducible Factor 1α (HIF1α) protein, a key activator of ANGPTL4. Our results demonstrate the pervasive downregulation of the specific HOTAIRM1 cytoplasmic isoform HM1-3 in ccRCC and suggest possible roles of HOTAIRM1 in kidney differentiation and suppression of HIF1-dependent angiogenic pathways.

Indexed as

Angiopoietin-Like Protein 4ApoptosisCarcinoma, Renal CellCell DifferentiationCell LineageCell Line, TumorCell ProliferationGene Expression Regulation, NeoplasticHumansHypoxia-Inducible Factor 1, alpha SubunitKidneyMicroRNAsProtein IsoformsSignal TransductionTumor HypoxiaAngiopoietin-Like Protein 4ANGPTL4 protein, humanHIF1A protein, humanHypoxia-Inducible Factor 1, alpha Subunitlong non-coding RNA HOTAIRM1, humanMicroRNAsProtein IsoformsccRCCCell differentiationHypoxia-Inducible Factor 1α (HIF1α)Kidney lineageLong non-coding RNA

Identifiers

PMID31862408
PMCPMC6992348
OpenAlexW2996166602

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.