Evidence map›Paper›PMID 31861889›Full record

ArticleBrain sciences2019

Selection of Reliable Reference Genes for Analysis of Gene Expression in Spinal Cord during Rat Postnatal Development and after Injury.

Ján Košuth, Martina Farkašovská, Filip Mochnacký, Zuzana Daxnerová, Juraj Ševc

Open access · goldAbstract read
In one paragraph

Article in Brain sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 21 citations in OpenAlex.

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  6. Implication of system xJournal of neuroinflammation · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ján KošuthInstitute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice, Šrobárova 2, 04154 Košice, Slovakia.
Martina FarkašovskáInstitute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice, Šrobárova 2, 04154 Košice, Slovakia.
Filip MochnackýInstitute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice, Šrobárova 2, 04154 Košice, Slovakia.
Zuzana DaxnerováInstitute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice, Šrobárova 2, 04154 Košice, Slovakia.
Juraj ŠevcInstitute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice, Šrobárova 2, 04154 Košice, Slovakia.
University of Pavol Jozef Šafárik · SK

Funding

Agentúra na podporu výskumu a vývoja APVV-15-0239Vedecká grantová agentúra MŠVVaŠ SR a SAV VEGA 1/0820/17
6 · The paper itself

Abstract

In order to obtain unbiased results of target gene expression, selection of the most appropriate reference gene (RG) remains a key precondition. However, an experimental study focused on the validation of stably expressed RGs in the rat spinal cord (SC) during development or after spinal cord injury (SCI) is missing. In our study, we tested the stability of the expression of nine selected RGs in rat SC tissue during normal development (postnatal days 1-43, adulthood) and after minimal (mSCI) and contusion (cSCI) spinal cord injury. The following RGs were tested: common housekeeping genes of basal cell metabolism (

Indexed as

normalizationpostnatal developmentreference geneRT-qPCRspinal cordspinal cord injury

Identifiers

PMID31861889
PMCPMC7017034
OpenAlexW2995445969

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.