ArticlePLoS genetics2019
Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Neuronal RNAi and oxygen-sensing circuit shape germline resilience to heat stress.Current biology : CB · 2026Article
- Small RNA pathways in mammalian oocytes.FEBS open bio · 2026Review
- Transposable element-gene chimera cartography, origination and role in enhancing transcriptome plasticity.Nature structural & molecular biology · 2026Article
- Enhanced RNAi does not provide efficient innate antiviral immunity in mice.Nucleic acids research · 2025Article
- Functional canonical RNAi in mice expressing a truncated Dicer isoform and long dsRNA.EMBO reports · 2024Article
- Themes and variations on piRNA-guided transposon control.Mobile DNA · 2023Review
- Structural and functional basis of mammalian microRNA biogenesis by Dicer.Molecular cell · 2022Article
- Revisiting ZAR proteins: the understudied regulator of female fertility and beyond.Cellular and molecular life sciences : CMLS · 2022Review
- piRNAs and PIWI Proteins as Diagnostic and Prognostic Markers of Genitourinary Cancers.Biomolecules · 2022Review
- Mouse Oocytes, A Complex Single Cell Transcriptome.Frontiers in cell and developmental biology · 2022Article
- Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets.International journal of molecular sciences · 2021Review
- Small Noncoding RNAs in Reproduction and Infertility.Biomedicines · 2021Review
- Small RNAs in epigenetic inheritance: from mechanisms to trait transmission.FEBS letters · 2021Review
- Review
- Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs.Nature cell biology · 2021Article
- The piRNA pathway is essential for generating functional oocytes in golden hamsters.Nature cell biology · 2021Article
- Small RNA expression and miRNA modification dynamics in human oocytes and early embryos.Genome research · 2021Article
- Global profiling of RNA-binding protein target sites by LACE-seq.Nature cell biology · 2021Article
- Piwi-interacting RNAs (piRNAs) as potential biomarkers and therapeutic targets for cardiovascular diseases.Angiogenesis · 2021Review
- Vitamin K in Vertebrates' Reproduction: Further Puzzling Pieces of Evidence from Teleost Fish Species.Biomolecules · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germline genome defense evolves to recognize and suppress retrotransposons. One of defensive mechanisms is the PIWI-associated RNA (piRNA) pathway, which employs small RNAs for sequence-specific repression. The loss of the piRNA pathway in mice causes male sterility while females remain fertile. Unlike spermatogenic cells, mouse oocytes posses also RNA interference (RNAi), another small RNA pathway capable of retrotransposon suppression. To examine whether RNAi compensates the loss of the piRNA pathway, we produced a new RNAi pathway mutant DicerSOM and crossed it with a catalytically-dead mutant of Mili, an essential piRNA gene. Normal follicular and oocyte development in double mutants showed that RNAi does not suppress a strong ovarian piRNA knock-out phenotype. However, we observed redundant and non-redundant targeting of specific retrotransposon families illustrating stochasticity of recognition and targeting of invading retrotransposons. Intracisternal A Particle retrotransposon was mainly targeted by the piRNA pathway, MaLR and RLTR10 retrotransposons were targeted mainly by RNAi. Double mutants showed accumulations of LINE-1 retrotransposon transcripts. However, we did not find strong evidence for transcriptional activation and mobilization of retrotransposition competent LINE-1 elements suggesting that while both defense pathways are simultaneously expendable for ovarian oocyte development, yet another transcriptional silencing mechanism prevents mobilization of LINE-1 elements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.