ArticleBioscience reports2020
Insights into the Cnx1E catalyzed MPT-AMP hydrolysis.
Article in Bioscience reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Altered transposon element-derived genes distort oxygen-free radical scavenger systems in FXD.NAR molecular medicine · 2026Article
- Convergent evolution links molybdenum insertase domains with organism-specific sequences.Communications biology · 2024Article
- The Final Step in Molybdenum Cofactor Biosynthesis-A Historical View.Molecules (Basel, Switzerland) · 2024Review
- Advancing Our Understanding of Pyranopterin-Dithiolene Contributions to Moco Enzyme Catalysis.Molecules (Basel, Switzerland) · 2023Review
- The structural principles underlying molybdenum insertase complex assembly.Protein science : a publication of the Protein Society · 2023Article
- Function of Molybdenum Insertases.Molecules (Basel, Switzerland) · 2022Review
- The History of the Molybdenum Cofactor-A Personal View.Molecules (Basel, Switzerland) · 2022Review
- Characterization of the nickel-inserting cyclometallase LarC from Moorella thermoacetica and identification of a cytidinylylated reaction intermediate.Metallomics : integrated biometal science · 2022Article
- Mechanism of molybdate insertion into pterin-based molybdenum cofactors.Nature chemistry · 2021Article
- Protein-bound molybdenum cofactor is bioavailable and rescues molybdenum cofactor-deficientGenes & development · 2021Article
- Identification and characterisation of the Volvox carteri Moco carrier protein.Bioscience reports · 2020Article
- Biological pincer complexes.ChemCatChem · 2020Article
- The structure of the Moco carrier protein from Rippkaea orientalis.Acta crystallographica. Section F, Structural biology communications · 2020Article
- The First Step ofMicroorganisms · 2020Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molybdenum insertases (Mo-insertases) catalyze the final step of molybdenum cofactor (Moco) biosynthesis, an evolutionary old and highly conserved multi-step pathway. In the first step of the pathway, GTP serves as substrate for the formation of cyclic pyranopterin monophosphate, which is subsequently converted into molybdopterin (MPT) in the second pathway step. In the following synthesis steps, MPT is adenylated yielding MPT-AMP that is subsequently used as substrate for enzyme catalyzed molybdate insertion. Molybdate insertion and MPT-AMP hydrolysis are catalyzed by the Mo-insertase E-domain. Earlier work reported a highly conserved aspartate residue to be essential for Mo-insertase functionality. In this work, we confirmed the mechanistic relevance of this residue for the Arabidopsis thaliana Mo-insertase Cnx1E. We found that the conservative substitution of Cnx1E residue Asp274 by Glu (D274E) leads to an arrest of MPT-AMP hydrolysis and hence to the accumulation of MPT-AMP. We further showed that the MPT-AMP accumulation goes in hand with the accumulation of molybdate. By crystallization and structure determination of the Cnx1E variant D274E, we identified the potential reason for the missing hydrolysis activity in the disorder of the region spanning amino acids 269 to 274. We reasoned that this is caused by the inability of a glutamate in position 274 to coordinate the octahedral Mg2+-water complex in the Cnx1E active site.
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