ArticleBiomaterials science2020
Enzymatically crosslinked gelatin-laminin hydrogels for applications in neuromuscular tissue engineering.
Article in Biomaterials science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 60 citations in OpenAlex.
- A Comprehensive Review on Food-Grade Electrospinning of Natural Biopolymers for Cultivated Meat Applications.Foods (Basel, Switzerland) · 2026Review
- Characterisation and optimisation of microbial production of transglutaminase produced by Streptoverticillium cinnamoneum.Applied microbiology and biotechnology · 2025Article
- Spheroid Cell Aggregation Enhanced by Enzyme-Free Ultrasound-Detached Cells.Advanced biology · 2025Article
- Dual Role of Ibuprofen and Indomethacin in Promoting Peripheral Nerve RegenerationTissue engineering. Part A · 2025Article
- Extracellular matrix in skeletal muscle injury and atrophy: mechanisms and therapeutic implications.Journal of orthopaedic translation · 2025Review
- Filamented hydrogels as tunable conduits for guiding neurite outgrowth.Materials today. Bio · 2025Article
- Mesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures?Bioengineering (Basel, Switzerland) · 2024Article
- Nanomedicine in Neuroprotection, Neuroregeneration, and Blood-Brain Barrier Modulation: A Narrative Review.Medicina (Kaunas, Lithuania) · 2024Review
- Engineering programmable material-to-cell pathways via synthetic notch receptors to spatially control differentiation in multicellular constructs.Nature communications · 2024Article
- Roadblocks confronting widespread dissemination and deployment of Organs on Chips.Nature communications · 2024Review
- Hierarchical Design of Tissue-Mimetic Fibrillar Hydrogel Scaffolds.Advanced healthcare materials · 2024Review
- Progress in the Use of Hydrogels for Antioxidant Delivery in Skin Wounds.Pharmaceutics · 2024Review
- Magnetic Chitosan Bionanocomposite Films as a Versatile Platform for Biomedical Hyperthermia.Advanced healthcare materials · 2024Article
- Tumor-on-a-chip models combined with mini-tissues or organoids for engineering tumor tissues.Theranostics · 2024Review
- Article
- Fabrication of a Gelatin-Based Microdevice for Vascular Cell Culture.Micromachines · 2022Article
- Optimization of Application-Driven Development ofTissue engineering. Part B, Reviews · 2022Review
- Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment.International journal of molecular sciences · 2022Article
- Exploring Biomolecular Self-Assembly with Far-Infrared Radiation.Biomolecules · 2022Review
- Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.ACS biomaterials science & engineering · 2022Article
Corrections and comments
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Authors and funding
15 authors at 1 institution in 3 countries.
Funding
Abstract
We report a water-soluble and non-toxic method to incorporate additional extracellular matrix proteins into gelatin hydrogels, while obviating the use of chemical crosslinkers such as glutaraldehyde. Gelatin hydrogels were fabricated using a range of gelatin concentrations (4%-10%) that corresponded to elastic moduli of approximately 1 kPa-25 kPa, respectively, a substrate stiffness relevant for multiple cell types. Microbial transglutaminase was then used to enzymatically crosslink a layer of laminin on top of gelatin hydrogels, resulting in 2-component gelatin-laminin hydrogels. Human induced pluripotent stem cell derived spinal spheroids readily adhered and rapidly extended axons on GEL-LN hydrogels. Axons displayed a more mature morphology and superior electrophysiological properties on GEL-LN hydrogels compared to the controls. Schwann cells on GEL-LN hydrogels adhered and proliferated normally, displayed a healthy morphology, and maintained the expression of Schwann cell specific markers. Lastly, skeletal muscle cells on GEL-LN hydrogels achieved long-term culture for up to 28 days without delamination, while expressing higher levels of terminal genes including myosin heavy chain, MyoD, MuSK, and M-cadherin suggesting enhanced maturation potential and myotube formation compared to the controls. Future studies will employ the superior culture outcomes of this hybrid substrate for engineering functional neuromuscular junctions and related organ on a chip applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.