Evidence map›Paper›PMID 31858380›Full record

ArticleMolecular and cellular biochemistry2020

Bone marrow mesenchymal stem cell-secreted exosomes carrying microRNA-125b protect against myocardial ischemia reperfusion injury via targeting SIRT7.

Qi Chen, Yu Liu, Xueyan Ding, Qinfeng Li, Fuyu Qiu, Meihui Wang, Zhida Shen, Hao Zheng, Guosheng Fu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
85citing papers in PubMed, 1 pooled it
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

85 citing papers in PubMed, 1 synthesis or guideline pooled it, 142 citations in OpenAlex.

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25 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Qi ChenDepartment of Cardiology, School of Medicine, Sir Run Run Shaw Hospital, Biomedical Research Center, Zhejiang University, No. 3, East Qingchun Road, Hangzhou, 310016, Zhejiang, China.
Yu LiuDepartment of Cardiology, Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital, No. 321, Zhongshan Road, Nanjing, 210008, Jiangsu, China.
Xueyan DingDepartment of Cardiology, Zhejiang Provincial People's Hospital, No. 158, Shangtang Road, Hangzhou, 310014, Zhejiang, China.
Qinfeng LiDepartment of Cardiology, School of Medicine, Sir Run Run Shaw Hospital, Biomedical Research Center, Zhejiang University, No. 3, East Qingchun Road, Hangzhou, 310016, Zhejiang, China.
Fuyu QiuDepartment of Cardiology, School of Medicine, Sir Run Run Shaw Hospital, Biomedical Research Center, Zhejiang University, No. 3, East Qingchun Road, Hangzhou, 310016, Zhejiang, China.
Meihui WangDepartment of Cardiology, School of Medicine, Sir Run Run Shaw Hospital, Biomedical Research Center, Zhejiang University, No. 3, East Qingchun Road, Hangzhou, 310016, Zhejiang, China.
Zhida ShenDepartment of Cardiology, School of Medicine, Sir Run Run Shaw Hospital, Biomedical Research Center, Zhejiang University, No. 3, East Qingchun Road, Hangzhou, 310016, Zhejiang, China.
Hao ZhengDepartment of Cardiology, Zhejiang Provincial People's Hospital, No. 158, Shangtang Road, Hangzhou, 310014, Zhejiang, China. zhenghao6169@163.com.
Guosheng FuDepartment of Cardiology, School of Medicine, Sir Run Run Shaw Hospital, Biomedical Research Center, Zhejiang University, No. 3, East Qingchun Road, Hangzhou, 310016, Zhejiang, China. fugs@zju.edu.cn.ORCID http://orcid.org/0000-0001-8559-6939
Sir Run Run Shaw Hospital · CNZhejiang Provincial People's Hospital · CNNanjing Drum Tower Hospital · CN

Funding

Effects and mechanisms of microRNA and O-GlcNAcylation on paracrine function of diabetic endothelial progenitor cell Y18H020004The mechanism research identifing Oct4 upregulating Cripto-1 mediated osteoblast differentiation of valve interstitial cells during the pathogenesis of aortic valve calcification 81800342
6 · The paper itself

Abstract

MicroRNA-125b (miR-125b) reduces myocardial infarct area and restrains myocardial ischemia reperfusion injury (I/R). In this study, we aimed to investigate the effect of bone marrow mesenchymal stem cell (BMSC)-derived exosomes carrying miR-125b on I/R rats. The myocardial I/R model in rats was constructed by ligation of the left anterior descending coronary artery (LAD). Rats were randomly divided into I/R and Sham group. Lv-cel-miR-67 (control) or Lv-miR-125b was transfected into BMSCs. Exosomes were extracted from transfected BMSCs, and separately named BMSC-Exo-67, BMSC-Exo-125b, and BMSC-Exo. MTT assay and flow cytometry were used to detect the viability and apoptosis of I/R myocardium cells, respectively. The expression of cell apoptosis proteins and the levels of inflammatory factors were examined by Western blot and ELISA assay, respectively. The target relationship between miR-125b and SIRT7 was predicted by using StarBase3.0, and was confirmed by using dual-luciferase reporter gene assay. qRT-PCR, immunohistochemistry staining, and Western blot were used to evaluate the expression of SIRT7 in myocardium tissues in I/R rats. BMSC-derived exosomes were successfully isolated and identified by TEM and positive expression of CD9 and CD63. The expression of miR-125b was down-regulated in I/R myocardium tissues and cells. BMSC-Exo-125b significantly up-regulated miR-125b in I/R myocardium cells. The intervention of BMSC-Exo-125b significantly increased the cell viability, decreased the apoptotic ratio, down-regulated Bax and caspase-3, up-regulated Bcl-2, and decreased the levels of IL-1β, IL-6, and TNF-α in I/R myocardium cells. SIRT7 was a target of miR-125b, and BMSC-Exo-125b significantly down-regulated SIRT7 in myocardium cells. In addition, the injection of BMSC-Exo-125b alleviated the pathological damages and down-regulated SIRT7 in myocardium tissues of I/R rats. BMSC-derived exosomes carrying miR-125b protected against myocardial I/R by targeting SIRT7.

Indexed as

ExosomesMicroRNAsMyocardial Reperfusion InjurySirtuinsAnimalsApoptosisBone Marrow CellsCytokinesMaleMesenchymal Stem CellsRatsRats, Sprague-DawleyCytokinesMicroRNAsMIRN125 microRNA, ratSIRT7 protein, ratSirtuinsApoptosisExosomesInflammatory factorIschemia reperfusionmiR-125b

Identifiers

PMID31858380
PMCPMC6955239
OpenAlexW2996170603

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.