ArticleJournal of experimental botany2020
Lethality caused by ADP-glucose accumulation is suppressed by salt-induced carbon flux redirection in cyanobacteria.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- The Relationship Between the Gut Microbiome and the Aqua Cultured Fish, General Prospects Toward Fish Health: A Systematic Review.Applied biochemistry and biotechnology · 2025Pooled it
- Glycogen deficiency impairs diurnal energy metabolism and cell division in Synechocystis.Plant physiology · 2026Article
- The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803.FEBS letters · 2026Article
- A new Persister Enrichment Model inbioRxiv : the preprint server for biology · 2026Article
- Article
- Enhancing D-lactic acid production from methane through metabolic engineering of Methylomonas sp. DH-1.Microbial cell factories · 2025Article
- Double blocking of carbon metabolism causes a large increase of Calvin-Benson cycle compounds in cyanobacteria.Plant physiology · 2024Article
- Stress response requires an efficient connection between glycogen and central carbon metabolism by phosphoglucomutases in cyanobacteria.Journal of experimental botany · 2023Article
- Metabolic analysis of the regulatory mechanism of sugars on secondary flowering in Magnolia.BMC molecular and cell biology · 2022Article
- Rubisco regulation in response to altered carbon status in the cyanobacterium Synechococcus elongatus PCC 7942.Plant physiology · 2022Article
- Split NanoLuc technology allows quantitation of interactions between PII protein and its receptors with unprecedented sensitivity and reveals transient interactions.Scientific reports · 2021Article
- Magnesium Signaling in Plants.International journal of molecular sciences · 2021Review
- Photosynthetic Co-Production of Succinate and Ethylene in A Fast-Growing Cyanobacterium,Metabolites · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are widely distributed photosynthetic organisms. During the day they store carbon, mainly as glycogen, to provide the energy and carbon source they require for maintenance during the night. Here, we generate a mutant strain of the freshwater cyanobacterium Synechocystis sp. PCC 6803 lacking both glycogen synthases. This mutant has a lethal phenotype due to massive accumulation of ADP-glucose, the substrate of glycogen synthases. This accumulation leads to alterations in its photosynthetic capacity and a dramatic decrease in the adenylate energy charge of the cell to values as low as 0.1. Lack of ADP-glucose pyrophosphorylase, the enzyme responsible for ADP-glucose synthesis, or reintroduction of any of the glycogen synthases abolishes the lethal phenotype. Viability of the glycogen synthase mutant is also fully recovered in NaCl-supplemented medium, which redirects the surplus of ADP-glucose to synthesize the osmolite glucosylglycerol. This alternative metabolic sink also suppresses phenotypes associated with the defective response to nitrogen deprivation characteristic of glycogen-less mutants, restoring the capacity to degrade phycobiliproteins. Thus, our system is an excellent example of how inadequate management of the adenine nucleotide pools results in a lethal phenotype, and the influence of metabolic carbon flux in cell viability and fitness.
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