ArticleScientific reports2019
Comparison of Escherichia coli surface attachment methods for single-cell microscopy.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Single-molecule tracking in living microbial cells.Biophysics reports · 2025Article
- Rapid antimicrobial susceptibility testing using carbon screen printed electrodes in a microfluidic device.Scientific reports · 2025Article
- Environmental conditions define the energetics of bacterial dormancy and its antibiotic susceptibility.Biophysical journal · 2023Article
- Achieving Single-Molecule Tracking of Subcellular Regulation in Bacteria during Real-Time Environmental Perturbations.Analytical chemistry · 2023Article
- Immobilization of Polyethyleneimine (PEI) on Flat Surfaces and Nanoparticles Affects Its Ability to Disrupt Bacterial Membranes.Microorganisms · 2021Article
- Article
- Probing bacterial cell wall growth by tracing wall-anchored protein complexes.Nature communications · 2021Article
- A General Workflow for Characterization of Nernstian Dyes and Their Effects on Bacterial Physiology.Biophysical journal · 2020Article
- Single-Cell Bacterial Electrophysiology Reveals Mechanisms of Stress-Induced Damage.Biophysical journal · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For in vivo, single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial physiology. However, a systematic comparison of the effects these attachment methods have on the bacterial physiology is lacking. Here we present such a comparison for bacterium Escherichia coli, and assess the growth rate, size and intracellular pH of cells growing attached to different, commonly used, surfaces. We demonstrate that E. coli grow at the same rate, length and internal pH on all the tested surfaces when in the same growth medium. The result suggests that tested attachment methods can be used interchangeably when studying E. coli physiology.
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Registered trials
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