ArticleScientific reports2019
Novel multimodal MRI and MicroCT imaging approach to quantify angiogenesis and 3D vascular architecture of biomaterials.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
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- Vascularization and Bone Regeneration with 3D-Printed Composite Scaffolds in Rodent Critical-Size Calvarial Defects: Systematic Review.Journal of functional biomaterials · 2026Review
- Advances in novel biomaterials for cardiovascular tissue engineering and regenerative medicine.PeerJ · 2026Review
- Randomized controlled trial of immediate alveolar ridge preservation for bone and soft tissue aesthetics after tooth extraction.Frontiers in surgery · 2026Article
- 3D bio-printed scaffolds and smart implants: evaluating functional performance in animal surgery models.Annals of medicine and surgery (2012) · 2025Review
- Visualizing spatiotemporal pattern of vascularization by SWIR fluorescence imaging in a mouse model of perforator flap transplantation.Journal of nanobiotechnology · 2025Article
- Ultrasonic Coating of Poly(D,L-lactic acid)/Poly(lactic-co-glycolic acid) Electrospun Fibers with ZnO Nanoparticles to Increase Angiogenesis in the CAM Assay.Biomedicines · 2024Article
- Non-invasive evaluation of endometrial microvessels via in vivo intrauterine photoacoustic endoscopy.Photoacoustics · 2024Article
- Dynamic contrast-enhanced magnetic resonance imaging for monitoring neovascularization during bone regeneration-a randomized in vivo study in rabbits.Clinical oral investigations · 2021Article
- Review
- 3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model.Scientific reports · 2021Article
- Medical imaging of tissue engineering and regenerative medicine constructs.Biomaterials science · 2021Review
- Artificial Intelligence (AI) Based Analysis ofIn vivo (Athens, Greece)Article
- Evolving applications of the egg: chorioallantoic membrane assay andJournal of tissue engineeringReview
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative assessment of functional perfusion capacity and vessel architecture is critical when validating biomaterials for regenerative medicine purposes and requires high-tech analytical methods. Here, combining two clinically relevant imaging techniques, (magnetic resonance imaging; MRI and microcomputed tomography; MicroCT) and using the chorioallantoic membrane (CAM) assay, we present and validate a novel functional and morphological three-dimensional (3D) analysis strategy to study neovascularization in biomaterials relevant for bone regeneration. Using our new pump-assisted approach, the two scaffolds, Optimaix (laminar structure mimicking entities of the diaphysis) and DegraPol (highly porous resembling spongy bone), were shown to directly affect the architecture of the ingrowing neovasculature. Perfusion capacity (MRI) and total vessel volume (MicroCT) strongly correlated for both biomaterials, suggesting that our approach allows for a comprehensive evaluation of the vascularization pattern and efficiency of biomaterials. Being compliant with the 3R-principles (replacement, reduction and refinement), the well-established and easy-to-handle CAM model offers many advantages such as low costs, immune-incompetence and short experimental times with high-grade read-outs when compared to conventional animal models. Therefore, combined with our imaging-guided approach it represents a powerful tool to study angiogenesis in biomaterials.
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Registered trials
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