ArticleStem cell research & therapy2019
Pre-aggregation of scalp progenitor dermal and epidermal stem cells activates the WNT pathway and promotes hair follicle formation in in vitro and in vivo systems.
Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Hair follicle organoids: advances in construction, applications, and translational challenges.Frontiers in cell and developmental biology · 2026Review
- A Bioengineered Skin Organoid Platform for Modeling Human Skin Physiology and Cytotoxicity.Research square · 2025Article
- Emerging biomedical engineering strategies for hair follicle regeneration.Bioactive materials · 2025Review
- Review
- Construction of Large-Scale Bioengineered Hair Germs and In Vivo Transplantation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Multi-omics and AI-driven advances in miRNA-mediated hair follicle regulation in cashmere goats.Frontiers in veterinary science · 2025Review
- Organoids in skin wound healing.Burns & trauma · 2025Review
- Hair Regeneration Methods Using Cells Derived from Human Hair Follicles and Challenges to Overcome.Cells · 2024Review
- Materials-based hair follicle engineering: Basic components and recent advances.Materials today. Bio · 2024Review
- Cocktail Cell-Reprogrammed Hydrogel Microspheres Achieving Scarless Hair Follicle Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Potential application of PBM use in hair follicle organoid culture for the treatment of androgenic alopecia.Materials today. Bio · 2023Review
- Bioengineered skin organoids: from development to applications.Military Medical Research · 2023Review
- In vitro hair follicle growth model for drug testing.Scientific reports · 2023Article
- A Kaleidoscope of Keratin Gene Expression and the Mosaic of Its Regulatory Mechanisms.International journal of molecular sciences · 2023Review
- The dermal papilla dilemma and potential breakthroughs in bioengineering hair follicles.Cell and tissue research · 2023Review
- TSF/FHA induces osteogenic differentiation of Mc3t3 cells via Pygo2 dependent Wnt/β-catenin signaling pathway.American journal of translational research · 2023Article
- Biofabrication of Human Skin with Its Appendages.Advanced healthcare materials · 2022Review
- Self-assembled complete hair follicle organoids by coculture of neonatal mouse epidermal cells and dermal cells in Matrigel.Annals of translational medicine · 2022Article
- The potential role of hsa_circ_0001079 in androgenetic alopecia via sponging hsa-miR-136-5p.Journal of clinical laboratory analysis · 2022Article
- Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling.Bioactive materials · 2022Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBillions of dollars are invested annually by pharmaceutical companies in search of new options for treating hair loss conditions; nevertheless, the challenge remains. One major limitation to hair follicle research is the lack of effective and efficient drug screening systems using human cells. Organoids, three-dimensional in vitro structures derived from stem cells, provide new opportunities for studying organ development, tissue regeneration, and disease pathogenesis. The present study focuses on the formation of human hair follicle organoids.
methodsScalp-derived dermal progenitor cells mixed with foreskin-derived epidermal stem cells at a 2:1 ratio aggregated in suspension to form hair follicle-like organoids, which were confirmed by immunostaining of hair follicle markers and by molecular dye labeling assays to analyze dermal and epidermal cell organization in those organoids. The hair-forming potential of organoids was examined using an in vivo transplantation assay.
resultsPre-aggregation of dermal and epidermal cells enhanced hair follicle formation in vivo. In vitro pre-aggregation initiated the interactions of epidermal and dermal progenitor cells resulting in activation of the WNT pathway and the formation of pear-shape structures, named type I aggregates. Cell-tracing analysis showed that the dermal and epidermal cells self-assembled into distinct epidermal and dermal compartments. Histologically, the type I aggregates expressed early hair follicle markers, suggesting the hair peg-like phase of hair follicle morphogenesis. The addition of recombinant WNT3a protein to the medium enhanced the formation of these aggregates, and the Wnt effect could be blocked by the WNT inhibitor, IWP2.
conclusionsIn summary, our system supports the rapid formation of a large number of hair follicle organoids (type I aggregates). This system provides a platform for studying epithelial-mesenchymal interactions, for assessing inductive hair stem cells and for screening compounds that support hair follicle regeneration.
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