ArticleAngewandte Chemie (International ed. in English)2020
Mapping Enzyme Activity on Tissue by Functional Mass Spectrometry Imaging.
Article in Angewandte Chemie (International ed. in English), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Progress in monitoring molecular, cellular and metabolic dynamics using mass spectrometry imaging.Bioanalysis · 2025Review
- The gene regulatory mechanisms shaping the heterogeneity of venom production in the Cape coral snake.Genome biology · 2025Article
- Cnidarian toxins: omics approaches and recombinant proteins.The journal of venomous animals and toxins including tropical diseases · 2025Article
- Myxospore density of Kudoa inornata varies significantly within symmetrical white muscle tissue replicates of its fish host, the spotted seatrout, Cynoscion nebulosus.Parasitology research · 2024Article
- Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications.Bone research · 2024Review
- Mapping enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons.Nature methods · 2024Article
- Ozone-enabled fatty acid discovery reveals unexpected diversity in the human lipidome.Nature communications · 2023Article
- Functional mass spectrometry imaging maps phospholipase-A2 enzyme activity during osteoarthritis progression.Theranostics · 2023Article
- Highlighting Functional Mass Spectrometry Imaging Methods in Bioanalysis.Journal of proteome research · 2022Review
- Physiological constraints dictate toxin spatial heterogeneity in snake venom glands.BMC biology · 2022Article
- Modern venomics-Current insights, novel methods, and future perspectives in biological and applied animal venom research.GigaScience · 2022Article
- Evolution, Expression Patterns, and Distribution of Novel Ribbon Worm Predatory and Defensive Toxins.Molecular biology and evolution · 2022Article
- Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling.Molecules (Basel, Switzerland) · 2022Article
- High-resolution imaging and identification of biomolecules using Nano-DESI coupled to ion mobility spectrometry.Analytica chimica acta · 2021Article
- Mass Spectrometry Imaging of Lipids with Isomer Resolution Using High-Pressure Ozone-Induced Dissociation.Analytical chemistry · 2021Article
- Review
- Ultrafast enzymatic digestion of deoxyribonucleic acid in aqueous microdroplets for sequence discrimination and identification.QRB discovery · 2021Article
- Multiplex enzyme activity imaging by MALDI-IMS of substrate library conversions.Scientific reports · 2020Article
- Characterising Functional Venom Profiles of Anthozoans and Medusozoans within Their Ecological Context.Marine drugs · 2020Review
- Mapping Enzyme Activity on Tissue by Functional Mass Spectrometry Imaging.Angewandte Chemie (International ed. in English) · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Enzymes are central components of most physiological processes, and are consequently implicated in various pathologies. High-resolution maps of enzyme activity within tissues therefore represent powerful tools for elucidating enzymatic functions in health and disease. Here, we present a novel mass spectrometry imaging (MSI) method for assaying the spatial distribution of enzymatic activity directly from tissue. MSI analysis of tissue sections exposed to phospholipid substrates produced high-resolution maps of phospholipase activity and specificity, which could subsequently be compared to histological images of the same section. Functional MSI thus represents a new and generalisable method for imaging biological activity in situ.
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Registered trials
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