ArticleMolecular biology reports2020
Wnt signaling regulates the proliferation potential and lineage commitment of human umbilical cord derived mesenchymal stem cells.
Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Chemotherapy-driven expression of WNT ligands in bone marrow stromal cells contributes to chemoresistance in acute lymphoblastic leukaemia.British journal of haematology · 2026Article
- Free-floating long-term vascularized mesenchymal organoids.iScience · 2026Article
- Identification and localization of telocyte-like cells in human umbilical cord stroma.Scientific reports · 2025Article
- Lithium chloride-treated human umbilical cord mesenchymal stem cells-derived exsomes promote hair growth by regulating miR-146a-5p-wnt pathway.Stem cells translational medicine · 2025Article
- Natural Polyphenols and Mesenchymal Stem Cells: A New Insight in Bone Regenerative Medicine.Stem cells international · 2025Review
- Signaling Pathways in Trans-differentiation of Mesenchymal Stem Cells: Recent Advances.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Simulation of Microgravity and Coculturing with Hematopoietic Cells Oppositely Modulate Wnt Signaling in Mesenchymal Stromal Cells.Doklady. Biochemistry and biophysics · 2023Article
- Editorial: Therapeutic potential of mesenchymal stem cells in organ and tissue regeneration.Frontiers in bioengineering and biotechnology · 2023Article
- Maternal inappropriate calcium intake aggravates dietary-induced obesity in male offspring by affecting the differentiation potential of mesenchymal stem cells.World journal of stem cells · 2022Article
- A Comprehensive Cancer-Associated MicroRNA Expression Profiling and Proteomic Analysis of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes.Tissue engineering and regenerative medicine · 2022Article
- Gene Expression Profiles of Human Mesenchymal Stromal Cells Derived from Wharton's Jelly and Amniotic Membrane before and after Osteo-Induction Using NanoString Platform.Current issues in molecular biology · 2022Article
- A Small-Molecule Cocktails-Based Strategy in Culture of Mesenchymal Stem Cells.Frontiers in bioengineering and biotechnology · 2022Article
- Tumor Suppressor microRNAs in Gastrointestinal Cancers: A Mini-Review.Recent advances in inflammation & allergy drug discovery · 2022Review
- The therapeutic potential of mesenchymal stem cells in treating osteoporosis.Biological research · 2021Review
- Current understanding of the mesenchymal stem cell-derived exosomes in cancer and aging.Biotechnology reports (Amsterdam, Netherlands) · 2021Review
- Inhibition versus activation of canonical Wnt-signaling, to promote chondrogenic differentiation of Mesenchymal Stem Cells. A review.Orthopedic reviews · 2021Review
- Running Against the Wnt: How Wnt/β-Catenin Suppresses Adipogenesis.Frontiers in cell and developmental biology · 2021Review
- The Role of Notch and Wnt Signaling in MSC Communication in Normal and Leukemic Bone Marrow Niche.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Relatively less is known about the interactions that tightly regulate the mesenchymal stem cells (MSCs) to maintain their pluripotency. Recent studies reports that Wnt proteins might play an important role in governing the MSC cell fate. In this study, we tested the hypothesis that Wnt proteins differentially regulate in vitro differentiation of human umbilical cord derived MSCs. Stromal cells from human umbilical cord (hUCMSCs) were isolated and treated with Wnt inhibitor/activator. FACS analysis of hUCMSCs for CD29, CD90, CD73, CD44, CD45 marker expression and gene expression of Wnt target genes and lineage specific genes were performed after Lithium Chloride (LiCl) and Quercetin treatment for 6 days. The cultured primary hUCMSCs demonstrated elevated MSC surface marker expression with clonogenic properties and differentiation potentials towards osteogenic, adipogenic and chondrogenic lineages. Downregulation in the expression of Wnt with Quercetin treatment was noted. LiCl treatment increased cellular proliferation but did not influence differentiation suggesting that the cells retain pluripotency whereas Quercetin treatment downregulated stemness markers, Wnt target gene expression and promoted osteogenesis as demonstrated by FACS analysis, calcium estimation and gene expression studies. Shift of differentiation potential after the inhibition of Wnt signaling by Quercetin was evident from the gene expression data and elevated calcium production, driving MSCs towards probable osteogenic lineage. The findings in particular are likely to open an interesting avenue of biomedical research, summarizing the impact of Wnt signaling on lineage commitment of MSCs.
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Registered trials
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