Evidence map›Paper›PMID 31853751›Full record

ArticleMarine biotechnology (New York, N.Y.)2020

Expression and Characterization of a Bright Far-red Fluorescent Protein from the Pink-Pigmented Tissues of Porites lobata.

Mary C Bridges, Cheryl M Woodley, Esther C Peters, Lisa A May, Sylvia B Galloway

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 5 institutions in 1 country.

Mary C BridgesGraduate Program in Marine Biology, College of Charleston, Charleston, SC, USA.ORCID http://orcid.org/0000-0003-1101-0848
Cheryl M WoodleyNational Centers for Coastal Ocean Science Charleston Laboratory, NOS, NOAA, Charleston, SC, USA. cheryl.woodley@noaa.gov.ORCID http://orcid.org/0000-0001-7532-2934
Esther C PetersDepartment of Environmental Science & Policy, George Mason University, Fairfax, VA, USA.ORCID http://orcid.org/0000-0001-5404-5309
Lisa A MayConsolidated Safety Services, Inc., NCCOS Charleston Laboratory, NOS, NOAA, Charleston, SC, USA.ORCID http://orcid.org/0000-0002-5050-1474
Sylvia B GallowayNational Centers for Coastal Ocean Science Charleston Laboratory, NOS, NOAA, Charleston, SC, USA.
Consolidated Safety Services-Dynamac (United States) · USGeorge Mason University · USMedical University of South Carolina · USNOAA National Centers for Coastal Ocean Science · USNOAA National Ocean Service · US

Funding

NOAA Coral Reef Conservation Program Project 1133
6 · The paper itself

Abstract

Members of the anthozoan green fluorescent protein (GFP) family display a diversity of photo-physical properties that can be associated with normal and damaged coral tissues. Poritid coral species often exhibit localized pink pigmentation in diseased or damaged tissues. Our spectral and histological analyses of pink-pigmented Porites lobata lesions show co-localization of bright red fluorescence with putative amoebocytes concentrating in the epidermis, suggesting an activated innate immune response. Here we report the cloning, expression, and characterization of a novel red fluorescent protein (plobRFP) from the pink-pigmented tissues associated with lesions on Porites lobata. In vitro, the recombinant plobRFP exhibits a distinct red emission signal of 614 nm (excitation maximum: 578 nm), making plobRFP the furthest red-shifted natural fluorescent protein isolated from a scleractinian coral. The recombinant protein has a high molar extinction coefficient (84,000 M

Indexed as

AnimalsAnthozoaGene Expression RegulationImmunity, InnateLuminescent ProteinsPlasmidsRecombinant ProteinsRed Fluorescent ProteinSequence Analysis, DNALuminescent ProteinsRecombinant ProteinsRed Fluorescent ProteinCoral innate immune responsePorites lobataRed fluorescent protein (RFP)tissue pigmentation response (TPR)

Identifiers

PMID31853751
OpenAlexW2995104246

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.