ArticleScience advances2019
Topology-dependent anomalous dynamics of ring and linear DNA are sensitive to cytoskeleton crosslinking.
Article in Science advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 0 citations in OpenAlex.
- Optical-Tweezers-integrating-Differential-Dynamic-Microscopy maps the spatiotemporal propagation of nonlinear strains in polymer blends and composites.Nature communications · 2022Article
- Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics.Journal of visualized experiments : JoVE · 2022Article
- Topological digestion drives time-varying rheology of entangled DNA fluids.Nature communications · 2022Article
- Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy.Journal of visualized experiments : JoVE · 2022Article
- Motor-driven advection competes with crowding to drive spatiotemporally heterogeneous transport in cytoskeleton composites.Frontiers in physics · 2022Article
- Topological tuning of DNA mobility in entangled solutions of supercoiled plasmids.Science advances · 2021Article
- Subtle changes in crosslinking drive diverse anomalous transport characteristics in actin-microtubule networks.Soft matter · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cytoskeletal crowding plays a key role in the diffusion of DNA molecules through the cell, acting as a barrier to effective intracellular transport and conformational stability required for processes such as transfection, viral infection, and gene therapy. Here, we elucidate the transport properties and conformational dynamics of linear and ring DNA molecules diffusing through entangled and crosslinked composite networks of actin and microtubules. We couple single-molecule conformational tracking with differential dynamic microscopy to reveal that ring and linear DNA exhibit unexpectedly distinct transport properties that are influenced differently by cytoskeleton crosslinking. Ring DNA coils are swollen and undergo heterogeneous and biphasic subdiffusion that is hindered by crosslinking. Conversely, crosslinking actually facilitates the single-mode subdiffusion that compacted linear chains exhibit. Our collective results demonstrate that transient threading by cytoskeleton filaments plays a key role in the dynamics of ring DNA, whereas the mobility of the cytoskeleton dictates transport of linear DNA.
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Registered trials
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