ArticleBiomedical optics express2019
Multi-plane, wide-field fluorescent microscopy for biodynamic imaging
Article in Biomedical optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.Biomedical optics express · 2026Article
- Multifocal fluorescence video-rate imaging of centimetre-wide arbitrarily shaped brain surfaces at micrometric resolution.Nature biomedical engineering · 2024Article
- Multiplane HiLo microscopy with speckle illumination and non-local means denoising.Journal of biomedical optics · 2023Article
- Experimental characterization, modelling and compensation of temperature effects in optotunable lenses.Scientific reports · 2023Article
- Fast volumetric imaging with line-scan confocal microscopy by electrically tunable lens at resonant frequency.Optics express · 2022Article
- Background inhibited and speed-loss-free volumetric imagingFrontiers in neuroscience · 2022Article
- Review
- Precompensation of 3D field distortions in remote focus two-photon microscopy.Biomedical optics express · 2021Article
- Real-time brain-wide multi-planar microscopy for simultaneous cortex and hippocampus imaging at the cellular resolution in mice.Biomedical optics express · 2021Article
- A Compact High-Speed Image-Based Method for Measuring the Longitudinal Motion of Living Tissues.Sensors (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wide-field fluorescent microscopy (WFFM) is widely employed in biomedical studies, due to its inherent advantages in high-speed imaging of biological dynamics noninvasively and specifically. However, WFFM suffers from the loss of axial resolution and the poor resistance to light scattering in deep tissue imaging. Here we propose a novel WFFM which has the capability in optical sectioning and volumetric imaging. We perform speckle illumination with a digital-micromirror-device for optical sectioning and employ an electrically tunable lens for defocusing modulation so as to quickly switch the image planes. We demonstrate its applications in multi-plane, wide-field imaging of biological dynamics in both zebrafish brains and mouse brains
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.