ArticleScientific reports2019
Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
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- SL-6 Mimic Is a Biostimulant forPlants (Basel, Switzerland) · 2025Article
- AlgaeOrtho, a bioinformatics tool for processing ortholog inference results in algae.Frontiers in microbiology · 2025Article
- Isolation and Selection of Protein-Rich Mutants ofPlants (Basel, Switzerland) · 2024Article
- Large-scale production of myco-fabricated ZnO/MnO nanocomposite using endophytic Colonstachys rosea with its antimicrobial efficacy against human pathogens.Scientific reports · 2024Article
- Comprehensive assessment of microalgal-based treatment processes for dairy wastewater.Frontiers in bioengineering and biotechnology · 2024Review
- Novel Strigolactone Mimics That Modulate Photosynthesis and Biomass Accumulation inMolecules (Basel, Switzerland) · 2023Article
- Optimization of Heterotrophic Culture Conditions for the AlgaePlants (Basel, Switzerland) · 2023Article
- A Low-Cost Fertilizer Medium Supplemented with Urea for the Lutein Production ofBioengineering (Basel, Switzerland) · 2023Article
- Biotechnological Approaches for Biomass and Lipid Production Using MicroalgaeJournal of microbiology and biotechnology · 2022Review
- Enhanced β-carotene and Biomass Production by Induced Mixotrophy inMetabolites · 2021Article
- Treatment of Synthetic Ammonium Sulfate Wastewater by Mixed Culture of Chlorella pyrenoidosa and Enriched Nitrobacteria.Current microbiology · 2021Article
- Insights into the physiology of Chlorella vulgaris cultivated in sweet sorghum bagasse hydrolysate for sustainable algal biomass and lipid production.Scientific reports · 2021Article
- Evaluation of Thirty Microalgal Isolates as Biodiesel Feedstocks Based on Lipid Productivity and Triacylglycerol (TAG) Content.Current microbiology · 2021Article
- Enhancement of Lipid Production under Heterotrophic Conditions by Overexpression of an Endogenous bZIP Transcription Factor inJournal of microbiology and biotechnology · 2020Article
- Plant anti-aging: Delayed flower and leaf senescence inPlant signaling & behavior · 2020Article
- Isolation and Characterization of Novel Chlorella Vulgaris Mutants With Low Chlorophyll and Improved Protein Contents for Food Applications.Frontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The heterotrophic cultivation of microalgae has a number of notable advantages, which include allowing high culture density levels as well as enabling the production of biomass in consistent and predictable quantities. In this study, the full potential of Chlorella sp. HS2 is explored through optimization of the parameters for its heterotrophic cultivation. First, carbon and nitrogen sources were screened in PhotobioBox. Initial screening using the Plackett-Burman design (PBD) was then adopted and the concentrations of the major nutrients (glucose, sodium nitrate, and dipotassium phosphate) were optimized via response surface methodology (RSM) with a central composite design (CCD). Upon validation of the model via flask-scale cultivation, the optimized BG11 medium was found to result in a three-fold improvement in biomass amounts, from 5.85 to 18.13 g/L, in comparison to a non-optimized BG11 medium containing 72 g/L glucose. Scaling up the cultivation to a 5-L fermenter resulted in a greatly improved biomass concentration of 35.3 g/L owing to more efficient oxygenation of the culture. In addition, phosphorus feeding fermentation was employed in an effort to address early depletion of phosphate, and a maximum biomass concentration of 42.95 g/L was achieved, with biomass productivity of 5.37 g/L/D.
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