ArticleScientific reports2019
Simultaneous detection and comprehensive analysis of HPV and microbiome status of a cervical liquid-based cytology sample using Nanopore MinION sequencing.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Evaluation of targeted next-generation sequencing for detection of HPV genotypes and sublineages in cervical liquid-based cytology SurePath samples from the Danish screening program.International journal of cancer · 2026Article
- Review
- Development and Evaluation of a MinION Full-Length 16S rDNA Sequencing Analysis Pipeline for Rapid Diagnosis of Animal Gastrointestinal Diseases.Microorganisms · 2025Article
- HPV integration profiling using nanopore sequencing and association with cervical precancerous lesion.Frontiers in microbiology · 2025Article
- Risk assessment and triage strategy of cervical cancer primary screening on HPV integration status: 5-year follow-up of a prospective cohort study.Journal of the National Cancer Center · 2024Article
- Evaluation of the cervical liquid-based cytology sample as a microbiome resource for dual diagnosis.PloS one · 2024Article
- The newest Oxford Nanopore R10.4.1 full-length 16S rRNA sequencing enables the accurate resolution of species-level microbial community profiling.Applied and environmental microbiology · 2023Article
- CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives.Journal of advanced research · 2023Review
- Detection of Microorganisms in Body Fluid Samples.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Establishment of early diagnosis models for cervical precancerous lesions using large-scale cervical cancer screening datasets.Virology journal · 2022Article
- Vaginal microbiota in ethnically diverse young women who did or did not develop pelvic inflammatory disease: community-based prospective study.Sexually transmitted infections · 2022Article
- Improving analysis of the vaginal microbiota of women undergoing assisted reproduction using nanopore sequencing.Journal of assisted reproduction and genetics · 2022Article
- Mixed Infection of Cytomegalovirus and Pulmonary Nocardiosis Caused by Nocardia elegans Diagnosed Using Nanopore Sequencing Technology.Internal medicine (Tokyo, Japan) · 2022Article
- MinION, a portable long-read sequencer, enables rapid vaginal microbiota analysis in a clinical setting.BMC medical genomics · 2022Article
- Application of third-generation sequencing in cancer research.Medical review (2021) · 2021Article
- Is Oxford Nanopore sequencing ready for analyzing complex microbiomes?FEMS microbiology ecology · 2021Article
- Accurate Detection of HPV Integration Sites in Cervical Cancer Samples Using the Nanopore MinION Sequencer Without Error Correction.Frontiers in genetics · 2020Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human papillomavirus (HPV) is a major pathogen that causes cervical cancer and many other related diseases. HPV infection related cervical microbiome could be an induce factor of cervical cancer. However, it is uncommon to find a single test on the market that can simultaneously provide information on both HPV and the microbiome. Herein, a novel method was developed in this study to simultaneously detect HPV infection and microbiota composition promptly and accurately. It provides a new and simple way to detect vaginal pathogen situation and also provide valuable information for clinical diagnose. This approach combined multiplex PCR, which targeted both HPV16 E6E7 and full-length 16S rRNA, and Nanopore sequencing to generate enough information to understand the vagina condition of patients. One HPV positive liquid-based cytology (LBC) sample was sequenced and analyzed. After comparing with Illumina sequencing, the results from Nanopore showed a similar microbiome composition. An instant sequencing evaluation showed that 15 min sequencing is enough to identify the top 10 most abundant bacteria. Moreover, two HPV integration sites were identified and verified by Sanger sequencing. This approach has many potential applications in pathogen detection and can potentially aid in providing a more rapid clinical diagnosis.
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Registered trials
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