Evidence map›Paper›PMID 31852766›Full record

ArticleJournal of clinical microbiology2020

Optimizing DNA Extraction Methods for Nanopore Sequencing of Neisseria gonorrhoeae Directly from Urine Samples.

Teresa L Street, Leanne Barker, Nicholas D Sanderson, James Kavanagh, Sarah Hoosdally, Kevin Cole, Robert Newnham, Mathyruban Selvaratnam, Monique Andersson, Martin J Llewelyn and 3 more

Open access · hybridAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 55 citations in OpenAlex.

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  10. Routine Metagenomics Service for ICU Patients with Respiratory Infection.American journal of respiratory and critical care medicine · 2024
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  13. Real-time genomics for One Health.Molecular systems biology · 2023
    Review
  14. Review
  15. Article
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  18. Article
  19. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

Teresa L StreetNuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom teresa.street@ndm.ox.ac.uk david.eyre@bdi.ox.ac.uk.
Leanne BarkerNuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.
Nicholas D SandersonNuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.
James KavanaghNuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.
Sarah HoosdallyNuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.
Kevin ColeDepartment of Microbiology and Infection, Royal Sussex County Hospital, Brighton, United Kingdom.
Robert NewnhamMicrobiology Laboratory, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom.
Mathyruban SelvaratnamMicrobiology Laboratory, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom.
Monique AnderssonMicrobiology Laboratory, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom.
Martin J LlewelynDepartment of Microbiology and Infection, Royal Sussex County Hospital, Brighton, United Kingdom.
Justin O'GradyQuadram Institute Bioscience, Norwich, United Kingdom.
Derrick W CrookNuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.
David W EyreNuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom teresa.street@ndm.ox.ac.uk david.eyre@bdi.ox.ac.uk.
University of Oxford · GBJohn Radcliffe Hospital · GBRoyal Sussex County Hospital · GBNational Institute for Health Research · GBQuadram Institute · GB

Funding

Department of Health RP-PG-0514-20018Medical Research Council MR/N013956/1
6 · The paper itself

Abstract

Empirical gonorrhea treatment at initial diagnosis reduces onward transmission. However, increasing resistance to multiple antibiotics may necessitate waiting for culture-based diagnostics to select an effective treatment. There is a need for same-day culture-free diagnostics that identify infection and detect antimicrobial resistance. We investigated if Nanopore sequencing can detect sufficient

Indexed as

Chlamydia InfectionsGonorrheaNanopore SequencingChlamydia trachomatisDNAHumansMaleNeisseria gonorrhoeaeDNADNA extractionNanopore sequencingNeisseria gonorrhoeaewhole-genome sequencing

Identifiers

PMID31852766
PMCPMC7041563
OpenAlexW2996018551

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.