Evidence map›Paper›PMID 31852667›Full record

ArticleBiology open2020

Use of in-gel peroxidase assay for cytochrome

Tsukasa Hara, Yuma Shibata, Ryosuke Amagai, Ayako Okado-Matsumoto

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Tsukasa HaraDepartment of Biology, Faculty of Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
Yuma ShibataDepartment of Biology, Faculty of Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
Ryosuke AmagaiDepartment of Biology, Faculty of Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
Ayako Okado-MatsumotoDepartment of Biology, Faculty of Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan ayako@bio.sci.toho-u.ac.jp.ORCID 0000-0003-0026-7453
Toho University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The in-gel activity assay (IGA) is a powerful technique that uses enzymatic activity and compares intensities of detected bands in mitochondrial respiratory chain supercomplexes, and it is applicable to eukaryotic organisms. However, no IGA has been established for complex III because of the difficulty of access by ubiquinol, a substrate for complex III. Herein, we demonstrate that cytochrome

Indexed as

AnimalsCytochromes cElectron Transport Complex IIIElectron Transport Complex IVElectrophoresis, Polyacrylamide GelEnzyme ActivationEnzyme AssaysMiceMitochondriaNative Polyacrylamide Gel ElectrophoresisOxidative PhosphorylationPeroxidaseRatsCytochromes cElectron Transport Complex IIIElectron Transport Complex IVPeroxidaseEnhanced chemiluminescenceHigh-resolution clear native polyacrylamide gel electrophoresisIn-gel activity assayMitochondrial respiratory chain complexesOxidative phosphorylationSupercomplex

Identifiers

PMID31852667
PMCPMC6955206
OpenAlexW2995025397

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.