Evidence map›Paper›PMID 31852666›Full record

ArticleBiology open2020

Defined extracellular ionic solutions to study and manipulate the cellular resting membrane potential.

Mattia Bonzanni, Samantha L Payne, Miryam Adelfio, David L Kaplan, Michael Levin, Madeleine J Oudin

Open access · goldAbstract read
In one paragraph

Article in Biology open, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Cancer: A bioelectric disease?Clinical and translational medicine · 2026
    Review
  3. Article
  4. Article
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  14. Article
  15. Balance of NaFrontiers in cell and developmental biology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Mattia BonzanniDepartment of Biomedical Engineering, Tufts University, Medford, 02155 MA, USA.
Samantha L PayneDepartment of Biomedical Engineering, Tufts University, Medford, 02155 MA, USA.ORCID 0000-0003-1256-0606
Miryam AdelfioDepartment of Biomedical Engineering, Tufts University, Medford, 02155 MA, USA.
David L KaplanDepartment of Biomedical Engineering, Tufts University, Medford, 02155 MA, USA.ORCID 0000-0002-9245-7774
Michael LevinAllen Discovery Center, Tufts University, Medford, 02155 MA, USA.ORCID 0000-0001-7292-8084
Madeleine J OudinDepartment of Biomedical Engineering, Tufts University, Medford, 02155 MA, USA madeleine.oudin@tufts.edu.ORCID 0000-0001-6988-4260
Tufts University · US

Funding

Engineering erythropoietin-producing cellsR24DK106743 · NIDDK · ROGOSIN INSTITUTE · PI CARROLL, THOMAS JOSEPH, CLEAVER, ONDINE B · 2015 to 2019
$7.0M
Investigating the role of the extracellular matrix in metastasis and chemo-resistanceR00CA207866 · NCI · TUFTS UNIVERSITY MEDFORD · PI OUDIN, MADELEINE JULIE · 2018 to 2020
$696k
NCI NIH HHS R00 CA207866NIDDK NIH HHS R24 DK106743
6 · The paper itself

Abstract

All cells possess an electric potential across their plasma membranes and can generate and receive bioelectric signals. The cellular resting membrane potential (RMP) can regulate cell proliferation, differentiation and apoptosis. Current approaches to measure the RMP rely on patch clamping, which is technically challenging, low-throughput and not widely available. It is therefore critical to develop simple strategies to measure, manipulate and characterize the RMP. Here, we present a simple methodology to study the RMP of non-excitable cells and characterize the contribution of individual ions to the RMP using a voltage-sensitive dye. We define protocols using extracellular solutions in which permeable ions (Na

Indexed as

AlgorithmsBiological TransportCell MembraneCell Membrane PermeabilityElectrophysiological PhenomenaEpithelial CellsExtracellular SpaceHumansIonsMembrane PotentialsModels, TheoreticalPatch-Clamp TechniquesSolutionsIonsSolutionsBioelectricityElectrophysiologyIonic solutionsNon-excitable cellsResting membrane potential

Identifiers

PMID31852666
PMCPMC6994931
OpenAlexW2995442551

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.