ArticlePloS one2019
Food from faeces: Evaluating the efficacy of scat DNA metabarcoding in dietary analyses.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- Using DNA Metabarcoding and Citizen Science to Assess the Diet of Owned Domestic Cats.Ecology and evolution · 2026Article
- Predictors and consequences of diet composition in a declining generalist aerial insectivore.Oecologia · 2026Article
- DIY: A Practical Field-to-Sequencer Workflow for Metabarcoding the Diet of Terrestrial Carnivore Species.Molecular ecology resources · 2026Article
- Diet and Prey Preference of Tigers (Ecology and evolution · 2026Article
- Combining Time-Stamped Insect Sampling With eDNA-Metabarcoding of Guano to Reconstruct Community Interactions.Ecology and evolution · 2026Article
- Article
- Revealing seasonal dietary niche overlap among sympatric large carnivores using DNA metabarcoding.Scientific reports · 2026Article
- Dietary DNA Metabarcoding From Animal Fecal Samples.Current protocols · 2026Article
- Integration of modern technologies to advance dietary assessment.Nature food · 2026Review
- DNA metabarcoding on roadkill stomach contents reveals the breadth of species present in bobcat diets.PloS one · 2026Article
- Evaluating the Quantitative Accuracy and Application of DNA Metabarcoding for Dietary Reconstruction in Ruminants.Ecology and evolution · 2026Article
- Dietary Response of Black-Backed Jackals (Ecology and evolution · 2025Article
- Metabarcoding provides accurate estimation of volumetric diet composition in a top predator despite interference from blocking primers.Scientific reports · 2025Article
- Identification of mammal species preyed upon by urban foxes (Vulpes vulpes) in Sapporo, Japan, determined by fecal DNA analysis.The Journal of veterinary medical science · 2025Article
- Range-Wide Assessment of the Tasmanian Devil Gut Microbiome.Ecology and evolution · 2025Article
- The InBIO Barcoding Initiative Database: contribution to the knowledge of DNA barcodes of the vascular plants of north-eastern Portugal.Biodiversity data journal · 2025Article
- Seasonal changes in invertebrate diet of breeding black-necked cranes (Ecology and evolution · 2024Article
- Molecular analysis of scats revealed diet and prey choice of grey wolves and Eurasian lynx in the contact zone between the Dinaric Mountains and the Alps.Frontiers in zoology · 2024Article
- DNA metabarcoding the diet ofCurrent zoology · 2023Article
- Contaminants reach everywhere: Fish dietary samples should be surface decontaminated prior to molecular diet analysis.Ecology and evolution · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Scat DNA metabarcoding is increasingly being used to track the feeding ecology of elusive wildlife species. This approach has greatly increased the resolution and detection success of prey items contained in scats when compared with other classical methods. However, there have been few studies that have systematically tested the applicability and reliability of this approach to study the diet of large felids species in the wild. Here we assessed the effectiveness of this approach in the cheetah Acinonyx jubatus. We tested how scat degradation, meal size, prey species consumed and feeding day (the day a particular prey was consumed) influenced prey DNA detection success in captive cheetahs. We demonstrated that it is possible to obtain diet information from 60-day old scats using genetic approaches, but the efficiency decreased over time. Probability of species-identification was highest for food items consumed one day prior to scat collection and the probability of being able to identify the species consumed increased with the proportion of the prey consumed. Detection success varied among prey species but not by individual cheetah. Identification of prey species using DNA detection methods from a single consumption event worked for samples collected between 8 and 72 hours post-feeding. Our approach confirms the utility of genetic approaches to identify prey species in scats and highlight the need to account for the systematic bias in results to control for possible scat degradation, feeding day, meal size and prey species consumed especially in the wild-collected scats.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.