ArticleAnalytical chemistry2020
Molecular Orientation Determination in Nanodiscs at the Single-Molecule Level.
Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 8 citations in OpenAlex.
- Nanodisc single-molecule pulldown to study lipid-protein interactions.Journal of lipid research · 2025Article
- Nanodiscs for the study of membrane proteins.Current opinion in structural biology · 2024Review
- Multiple mechanisms of self-association of chemokine receptors CXCR4 and CCR5 demonstrated by deep mutagenesis.bioRxiv : the preprint server for biology · 2023Article
- Ultrafast energy transfer between lipid-linked chromophores and plant light-harvesting complex II.Physical chemistry chemical physics : PCCP · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The function of membrane-bound proteins often depends on their interactions with the lipid bilayer. Bulk absorption-based linear dichroism has been historically used to investigate molecular orientations in the phospholipid bilayer but cannot resolve the actual distribution of molecules embedded in the membrane and is often limited by a poor signal-to-noise ratio. Here, we present single-molecule orientation determination by fluorescence-detected linear dichroism visualization in Nanodisc grids or SOLVING, to determine the molecular orientation of molecules assembled into nanoscale lipid bilayers. We provide a proof-of-concept by using SOLVING to quantitate the orientation distribution of two commonly used fluorescent dyes, DiO and BODIPY, in 10 nm Nanodiscs. Besides confirming the mean orientation determined by bulk absorption measurement, SOLVING provides the actual distribution of orientations and promises to provide key molecular insights into the topology and interactions of multiprotein complexes, such as those observed in intracellular signal transduction.
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Registered trials
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