ArticleFrontiers in bioengineering and biotechnology2019
Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid.
Article in Frontiers in bioengineering and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 14 citations in OpenAlex.
- Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation.Food chemistry: X · 2025Article
- Article
- Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Characterization of Protocatechuate 4,5-Dioxygenase fromInternational journal of molecular sciences · 2021Article
- Biological Materials: The Next Frontier for Cell-Free Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Review
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3 authors at 1 institution in 1 country.
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Abstract
The coffee industry produces over 10 billion kg beans per year and generates high amounts of different waste products. Spent coffee grounds (SCG) are an industrially underutilized waste resource, which is rich in the polysaccharide galactomannan, a polysaccharide consisting of a mannose backbone with galactose side groups. Here, we present a cell-free reaction cascade for the conversion of mannose, the most abundant sugar in SCG, into L-lactic acid. The enzymatic conversion is based on a so far unknown oxidative mannose metabolism from
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