ArticleInternational journal of nanomedicine2019
Skin Toxicity Assessment of Silver Nanoparticles in a 3D Epidermal Model Compared to 2D Keratinocytes.
Article in International journal of nanomedicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.
- Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies.Particle and fibre toxicology · 2020Pooled it
- New approach methodologies in nanomedicine: bridging the gap betweenChemical Society reviews · 2026Review
- Integrated Metabolomics and Multilevel Validation of the Anti-Inflammatory and Skin-Soothing Effects of Bioactive Components fromInternational journal of molecular sciences · 2026Article
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- The advancements in organoids: Potential and challenges in researching the esophagus and esophageal squamous cell carcinoma.Genes & diseases · 2026Review
- Global Trends and Hotspot Analysis of Nanomaterials in the Field of Acne: A Bibliometric Perspective.Clinical, cosmetic and investigational dermatology · 2026Article
- Efficacy Evaluation of N-Acyl-Phytosphingosine Prepared from Marula Oil Derived Fatty Acids in Skin Barrier Repair.Clinical, cosmetic and investigational dermatology · 2026Article
- Bridging the gap in Parkinson's disease research: from general pathophysiology to advanced 3d cell culture models.Cell communication and signaling : CCS · 2025Review
- Cinnamon-Mediated Silver Nanoparticles and Beta-Carotene Nanocarriers in Alginate Dressings for Wound Healing Applications.Gels (Basel, Switzerland) · 2025Article
- Cutaneous Evaluation of FeInternational journal of nanomedicine · 2025Article
- Citric acid is more effective than sodium thiosulfate in chelating calcium in a dissolution model of calcinosis.Scientific reports · 2024Article
- Multifunctional Ag-Poly(Polymers · 2024Article
- Evaluation of the safety and efficacy of skin penetration enhancer Putocrin®.Journal of cosmetic dermatology · 2024Article
- Nail Lacquer ContainingAntibiotics (Basel, Switzerland) · 2024Article
- Silver and Carbon Nanomaterials/Nanocomplexes as Safe and Effective ACE2-S Binding Blockers on Human Skin Cell Lines.Molecules (Basel, Switzerland) · 2024Article
- A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent.Biomedicines · 2024Article
- Recreating Human Skin In Vitro: Should the Microbiota Be Taken into Account?International journal of molecular sciences · 2024Review
- Recombinant Keratin-Chitosan Cryogel Decorated with Gallic Acid-Reduced Silver Nanoparticles for Wound Healing.International journal of nanomedicine · 2024Article
- Three-Dimensional Cell Cultures: The Bridge between In Vitro and In Vivo Models.International journal of molecular sciences · 2023Review
- Application of transgenic zebrafish for investigating inflammatory responses to nanomaterials: Recommendations for new users.F1000Research · 2023Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionIncreased use of silver nanoparticles (AgNPs) has raised concerns that AgNPs may induce toxic effects. In vitro studies of cell monolayers and in vivo studies have produced conflicting results. The inconsistency of these results has been mainly due to limitations of two-dimensional (2D) monolayer cell systems.
methodsA three-dimensional (3D) epidermal model called EpiKutis
resultsThe results demonstrated that cell viability was greater than 80% and lactate dehydrogenase (LDH) release did not increase even at the highest dose of AgNPs in EpiKutis. In contrast, treatment of 2D keratinocytes with AgNPs resulted in dose-dependent decrease in cell viability from 63% to 11%, and a dose-dependent increase in LDH release from 8% to 16%. Cytotoxicity of AgNPs in 2D keratinocytes was related to oxidative damage and inflammation, as evidenced by increased levels of reactive oxygen species (ROS), malondialdehyde (MDA), IL-1α, IL-6, and IL-8. In addition, levels of superoxide dismutase (SOD) were decreased. EpiKutis treated with AgNPs did not exhibit increased oxidative damage or inflammation, which may have been due to the barrier properties of the 3D structure, resulting in reduced penetration of AgNPs. At equivalent per cell doses, total silver penetration into EpiKutis was 0.9 ± 0.1%, and total silver penetration into 2D keratinocytes was 8.8 ± 0.6% detected by ICP-MS. The penetration and distribution of AgNPs in 2D keratinocytes were confirmed by the TEM-EDS analysis, which was not found in the 3D EpiKutis. These results showed that AgNPs penetrated EpiKutis to a lesser degree than they penetrated 2D keratinocytes, which suggested that EpiKutis exhibited significant barrier function. DISCUSSION: The results of this study showed that AgNP toxicity should be evaluated using 3D epidermal models, which may provide better estimates of in vivo conditions than 2D models. The EpiKutis model may be an ideal model for assessment of nanotoxicity.
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Registered trials
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