Evidence map›Paper›PMID 31849336›Full record

ArticleGenome biology2019

TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools.

Cécile Monat, Sudharsan Padmarasu, Thomas Lux, Thomas Wicker, Heidrun Gundlach, Axel Himmelbach, Jennifer Ens, Chengdao Li, Gary J Muehlbauer, Alan H Schulman and 8 more

Abstract readEvaluation Study
In one paragraph

Article in Genome biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 153 papers.

0numbers the graph read from it
0cells of the map it votes in
153citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

153 citing papers in PubMed.

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  10. Epistatic interaction of the genesbioRxiv : the preprint server for biology · 2026
    Article
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  15. INDETERMINATE DOMAIN-DELLA protein interactions orchestrate gibberellin-mediated cell elongation in wheat and barley.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  16. Exploring standing genetic variation for barley leaf rust resistance in Australian breeding panel.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Article
  17. Article
  18. Article
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  20. On the origin of the late-flowering ppd-H1 allele in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Article

93 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Cécile MonatLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.
Sudharsan PadmarasuLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.
Thomas LuxPGSB - Plant Genome and Systems Biology, Helmholtz Center Munich - German Research Center for Environmental Health, Neuherberg, Germany.
Thomas WickerDepartment of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
Heidrun GundlachPGSB - Plant Genome and Systems Biology, Helmholtz Center Munich - German Research Center for Environmental Health, Neuherberg, Germany.
Axel HimmelbachLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.
Jennifer EnsDepartment of Plant Sciences, University of Saskatchewan, Saskatoon, Canada.
Chengdao LiWestern Barley Genetics Alliance, School of Veterinary and Life Sciences (VLS), Murdoch University, Murdoch, WA, Australia.
Gary J MuehlbauerDepartment of Agronomy and Plant Genetics & Department of Plant and Microbial Biology, University of Minnesota, St. Paul, MN, USA.
Alan H SchulmanGreen Technology, Natural Resources Institute (Luke), Viikki Plant Science Centre, and Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
Robbie WaughThe James Hutton Institute, Dundee, UK.
Ilka BraumannCarlsberg Research Laboratory, Copenhagen, Denmark.
Curtis PozniakDepartment of Plant Sciences, University of Saskatchewan, Saskatoon, Canada.
Uwe ScholzLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.
Klaus F X MayerPGSB - Plant Genome and Systems Biology, Helmholtz Center Munich - German Research Center for Environmental Health, Neuherberg, Germany.
Manuel SpannaglPGSB - Plant Genome and Systems Biology, Helmholtz Center Munich - German Research Center for Environmental Health, Neuherberg, Germany.
Nils SteinLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany. stein@ipk-gatersleben.de.
Martin MascherLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany. mascher@ipk-gatersleben.de.ORCID 0000-0001-6373-6013

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chromosome-scale genome sequence assemblies underpin pan-genomic studies. Recent genome assembly efforts in the large-genome Triticeae crops wheat and barley have relied on the commercial closed-source assembly algorithm DeNovoMagic. We present TRITEX, an open-source computational workflow that combines paired-end, mate-pair, 10X Genomics linked-read with chromosome conformation capture sequencing data to construct sequence scaffolds with megabase-scale contiguity ordered into chromosomal pseudomolecules. We evaluate the performance of TRITEX on publicly available sequence data of tetraploid wild emmer and hexaploid bread wheat, and construct an improved annotated reference genome sequence assembly of the barley cultivar Morex as a community resource.

Indexed as

Chromosomes, PlantGenetic TechniquesGenome, PlantHordeumSoftwareTriticum

Identifiers

PMID31849336
PMCPMC6918601

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.