ArticleEMBO reports2020
A distinct metabolic state arises during the emergence of 2-cell-like cells.
Article in EMBO reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed.
- Generation of eight-cell embryo-like cells from human pluripotent stem cells.Nature protocols · 2026Review
- Chemical reprogramming directly resets mouse post-implantation epiblast cells to a totipotent state.Cell research · 2026Article
- Metabolic regulation of key developmental events during mammalian embryogenesis.Nature cell biology · 2025Review
- Article
- The ZBTB24-CDCA7-HELLS axis suppresses the totipotent 2C-like reprogramming by maintaining Dux methylation and repression.Nucleic acids research · 2025Article
- Lactylation of Hdac1 regulated by Ldh prevents the pluripotent-to-2C state conversion.Stem cell research & therapy · 2024Article
- Maternal factor Trim75 contributes to zygotic genome activation program in mouse early embryos.Molecular biology reports · 2024Article
- NELFA and BCL2 induce the 2C-like state in mouse embryonic stem cells in a chemically defined medium.Cell proliferation · 2024Article
- A comprehensive review: synergizing stem cell and embryonic development knowledge in mouse and human integrated stem cell-based embryo models.Frontiers in cell and developmental biology · 2024Review
- Advances in understanding the regulation of pluripotency fate transition in embryonic stem cells.Frontiers in cell and developmental biology · 2024Review
- Hyperosmotic stress induces 2-cell-like cells through ROS and ATR signaling.EMBO reports · 2023Article
- The transcription factor DUX4 orchestrates translational reprogramming by broadly suppressing translation efficiency and promoting expression of DUX4-induced mRNAs.PLoS biology · 2023Article
- A genome-wide screen reveals new regulators of the 2-cell-like cell state.Nature structural & molecular biology · 2023Article
- A critical role for heme synthesis and succinate in the regulation of pluripotent states transitions.eLife · 2023Article
- Multiple repeat regions within mouse DUX recruit chromatin regulators to facilitate an embryonic gene expression program.bioRxiv : the preprint server for biology · 2023Article
- 2-Cell-like Cells: An Avenue for Improving SCNT Efficiency.Biomolecules · 2022Review
- Hypoxia induces an early primitive streak signature, enhancing spontaneous elongation and lineage representation in gastruloids.Development (Cambridge, England) · 2022Article
- Exploration of nuclear body-enhanced sumoylation reveals that PML represses 2-cell features of embryonic stem cells.Nature communications · 2022Article
- TET1 regulates gene expression and repression of endogenous retroviruses independent of DNA demethylation.Nucleic acids research · 2022Article
- Genome architecture and totipotency: An intertwined relation during early embryonic development.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Pluripotent stem cells are thought of as a surrogate of early developmental stages that sustain the capacity to generate all cell types in the body, thereby constituting an invaluable tool to address the mechanisms underlying cellular plasticity. In the mouse, cells resembling totipotent 2-cell-stage embryos (2-cell-like cells) arise at a very low frequency in embryonic stem cell (ESC) cultures. However, the extent to which these early-embryonic-like cells recapitulate the molecular features of the early embryo is unclear. Here, we have undertaken a characterization of some of the metabolic features of early-embryonic-like cells in culture. Our data indicate that early-embryonic-like cells exhibit decreased glycolytic and respiratory activity, lower levels of reactive oxygen species and increased glucose uptake, suggesting a shift of the metabolic programme during 2-cell-like cell reprogramming. Accordingly, we find that 2-cell-like cells can be induced by defined metabolites. Thus, in addition to their transcriptional and chromatin features, 2-cell-like cells recapitulate some of the metabolic features of their in vivo counterpart. Altogether, our work underscores a distinct metabolic state of early-embryonic-like cells and identifies compounds that can induce their emergence in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.