Evidence map›Paper›PMID 31848917›Full record

ArticleMolecular biology reports2020

Cross-amplification of microsatellite loci in the cacti species from Brazilian Chaco.

Wellington Santos Fava, Vanessa G Nóbrega Gomes, Aline Pedroso Lorenz, Gecele Matos Paggi

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Characterizing the chloroplast genome ofMitochondrial DNA. Part B, Resources · 2023
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Wellington Santos FavaLaboratory of Genetics, Campus of Pantanal, Federal University of Mato Grosso do Sul, Corumbá, Mato Grosso do Sul, 79304-902, Brazil. weg.fava@gmail.com.ORCID http://orcid.org/0000-0002-3608-0503
Vanessa G Nóbrega GomesEcology and Conservation Graduate Program, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, 79070-900, Brazil.
Aline Pedroso LorenzLaboratory of Ecology and Evolutionary Biology, Institute of Biosciences, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, 79070-900, Brazil.
Gecele Matos PaggiLaboratory of Genetics, Campus of Pantanal, Federal University of Mato Grosso do Sul, Corumbá, Mato Grosso do Sul, 79304-902, Brazil.
Universidade Federal de Mato Grosso do Sul · BR

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (BR) Finance Code 001Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul Processo Fundect DCR-C: 59/300.248/2016: Termo de Outorga: 159/2016Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul SIAFEM: 026045Rufford Foundation RSGF 18851-1
6 · The paper itself

Abstract

Cactaceae species are an important component of the Brazilian Chaco landscape. Sixteen species are reported to this region, including 13 genera representing three Cactaceae subfamilies. All these species are native and have been locally threatened by the advance of the deforestation, which can negatively impact their genetic diversity. In order to test genetic markers that can potentially be used to screen the population diversity of these species, we checked the cross-amplification performance of 27 nuclear and 23 plastid microsatellite loci in all 16 cacti species from Brazilian Chaco. We tested the cross-amplification of the 50 microsatellite (SSR) loci in one specimen of each cacti species and considered it successful when at least one band of the expected size was generated. Thirteen species (81%) had at least 18 nuclear microsatellite loci amplified, while seven species (43%) had at least 11 chloroplast microsatellite loci amplified. We also reviewed current knowledge of SSR studies with Cactaceae in 50 studies available in the Web of Science

Indexed as

EcosystemBrazilCactaceaeMicrosatellite RepeatsPolymerase Chain ReactionSpecies SpecificityCactaceaeConservation geneticsEchinopsisPlastidial markersSSR markers

Identifiers

PMID31848917
OpenAlexW2996127160

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.