ArticleParasitology research2020
Extracellular protease profile of Acanthamoeba after prolonged axenic culture and after interaction with MDCK cells.
Article in Parasitology research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.
- A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review.Memorias do Instituto Oswaldo Cruz · 2022Pooled it
- Proteomic identification of Acanthamoeba castellanii excretory-secretory proteins associated with Klebsiella pneumoniae capsule enlargement: a candidate role for C-type lysozyme.Parasite (Paris, France) · 2026Article
- Development of a novel encystment medium: Enhancing diagnostic potential ofVeterinary world · 2025Article
- Assessment ofJournal of water and health · 2024Article
- Characterization of novel extracellular proteases produced by Acanthamoeba castellanii after contact with human corneal epithelial cells and their relevance to pathogenesis.Parasites & vectors · 2024Article
- Epidemiology of and Genetic Factors Associated withPathogens (Basel, Switzerland) · 2024Review
- Proteases of Acanthamoeba.Parasitology research · 2023Review
- Experimental keratitis induced in rat by Acanthamoeba from distinct morphological groups/genotypes: a histological and immunohistochemical evaluation.Parasitology research · 2023Article
- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- In Vitro Cytopathogenic Activities ofPathogens (Basel, Switzerland) · 2022Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Free-living amoebae of the genus Acanthamoeba are causative agents of Acanthamoeba keratitis and amoebic encephalitis in humans, both of which are serious infections. The ability to produce proteases is one of the factors involved in the pathogenesis of Acanthamoeba infections. The aim of this study was to evaluate the secreted proteases of six Acanthamoeba strains from distinct genotypes (T1, T2, T4 and T11) maintained in prolonged axenic culture and following three successive passages in Madin-Darby Canine Kidney (MDCK) cells. Conditioned medium was obtained from cultures before and after interaction with the MDCK monolayers, resolved in SDS-PAGE containing gelatine, then subjected to quantitative azocasein assays. Zymography profiles varied between the strains, with the predominant proteases found to be serine-type proteases from 49 to 128 kDa. A T1 genotype strain isolated from dust showed quantitatively higher protease secretion compared to the other strains. No changes were detected in the zymography profiles of MDCK-interacted cultures compared to long-term axenic cultures. Two strains presented lower proteolytic activity post-MDCK interaction, while the remaining strains presented similar values before and after MDCK passages. In conclusion, this study confirms the predominance of serine-type protease secretion by Acanthamoeba, with distinct profiles presented by the different strains and genotypes studied. Also, interaction of trophozoites with MDCK cells did not alter the zymography pattern.
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