ArticleScientific reports2019
Real-time PCR for direct aptamer quantification on functionalized graphene surfaces.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Investigation of the Impact of Thionine Functionalization on Magnetoelastic Sensor Performance.ACS applied bio materials · 2025Article
- The Development and Characterisation of ssDNA Aptamers via a Modified Cell-SELEX Methodology for the Detection of Human Red Blood Cells.International journal of molecular sciences · 2024Article
- Advances in diagnosis and control of anthelmintic resistant gastrointestinal helminths infecting ruminants.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection.Beilstein journal of nanotechnology · 2022Article
- Aptamer-based approaches forResearch in pharmaceutical sciences · 2020Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we develop a real-time PCR strategy to directly detect and quantify DNA aptamers on functionalized graphene surfaces using a Staphylococcus aureus aptamer (SA20) as demonstration case. We show that real-time PCR allowed aptamer quantification in the range of 0.05 fg to 2.5 ng. Using this quantitative technique, it was possible to determine that graphene functionalization with amino modified SA20 (preceded by a graphene surface modification with thionine) was much more efficient than the process using SA20 with a pyrene modification. We also demonstrated that the functionalization methods investigated were selective to graphene as compared to bare silicon dioxide surfaces. The precise quantification of aptamers immobilized on graphene surface was performed for the first time by molecular biology techniques, introducing a novel methodology of wide application.
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